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Association of allelic variation in two NPR1-like genes with Fusarium head blight resistance in wheat

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An Erratum to this article was published on 18 April 2014

Abstract

Fusarium head blight (FHB) is a destructive disease of wheat and barley. In wheat it is mainly caused by the fungal pathogens Fusarium graminearum and Fusarium culmorum. We report the identification and evaluation of candidate genes for quantitative FHB resistance. These genes showed altered expression levels in the moderately resistant winter wheat genotypes Capo and SVP72017 after inoculation with F. graminearum. Amongst others, a NPR1-like gene was identified. Sequence analysis of this gene fragment revealed a high level of variation between the parents of a doubled haploid population. Single nucleotide polymorphism and polymerase chain reaction markers were developed and two homoeologous genes were mapped on the long arms of chromosomes 2A and 2D, respectively. Markers for both genes had significant effects on FHB resistance in a diverse collection of 178 European winter wheat cultivars evaluated in multi-environmental field trials after spray inoculation with F. culmorum. These results revealed that allelic variation in two homoeologous NPR1-like genes is associated with FHB resistance in European winter wheat. Markers for these genes might therefore be used for marker-assisted breeding programs.

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Acknowledgments

We would like to thank S. Wüllner, S. Schmidt, J. Brandlmeier, A. Bund and the staff of the departments of Genome Analysis and Wheat Breeding of the Bavarian State Research Centre for excellent technical assistance. We thank V. Mohler, B. Büttner and T. Albrecht from the plant breeding department of the Bavarian State Research Centre for Agriculture for helpful discussions and critical reading of the manuscript. We are grateful to M. Lemmens and B. Steiner from the IFA Tulln, Austria for kindly providing the F. graminearum inoculum and for sharing their protocols and experience in inoculation and sampling technique. We acknowledge J. Schondelmaier from the Saaten-Union Biotec GmbH for performing the SSR marker analysis of the Sngh3559//Capo/SVP72017 mapping population. We also thank Saatzucht Breun GmbH & Co. KG (Herzogenaurach, Germany), Deutsche Saatveredelung AG (Leutewitz, Germany), Prof. H. Buerstmeyr from the Department for Agrobiotechnology Tulln (Austria) and W. von Borries-Eckendorf GmbH & Co. KG (Leopoldshöhe, Germany) for performing the field trials. Results presented here were obtained from a project funded by the Deutsche Forschungsgemeinschaft DFG (Schw 1201/1-2) and of the GABI-Canada (CGAT) cooperative project: Reducing Fusarium toxins in wheat through genomics (Subprojects A and C no. 0313711A and C), which was funded by the German Ministry of Education and Research.

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  1. Michael Schmolke

    Present address: Bayer CropScience AG, European Wheat Breeding Center, Am Schwabeplan 6, OT Gatersleben, 06466, Stadt Seeland, Germany

Authors and Affiliations

  1. Institute for Crop Science and Plant Breeding, Bavarian State Research Center for Agriculture, Am Gereuth 8, 85354, Freising, Germany

    Manuela Diethelm, Jennifer Groth, Günther Schweizer & Lorenz Hartl

  2. Plant Breeding, Technische Universität München, Emil-Ramann-Str.4, 85354, Freising, Germany

    Michael Schmolke

  3. Plant Breeding Department, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Wolfgang Friedt

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Correspondence to Manuela Diethelm.

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Diethelm, M., Schmolke, M., Groth, J. et al. Association of allelic variation in two NPR1-like genes with Fusarium head blight resistance in wheat. Mol Breeding 34, 31–43 (2014). https://doi.org/10.1007/s11032-013-0010-2

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