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Confirming quantitative trait loci for aflatoxin resistance from Mp313E in different genetic backgrounds

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Abstract

The fungus Aspergillus flavus (Link:Fr) causes ear rot of maize (Zea mays L.) and produces the toxic metabolic product aflatoxin. One particularly effective method of controlling the fungus is via host plant resistance, but while several resistant breeding lines have been identified, transferring the resistance genes from these lines into elite cultivars has been less effective than needed. A high number of genes involved with resistance, each with a small effect, and some only found under certain environmental conditions, has hampered resistance breeding. The identification of markers linked to genomic regions associated with resistance would aid in this effort. The goals of this study were to identify and characterize quantitative trait loci (QTL) conferring resistance to aflatoxin accumulation from resistant maize donor Mp313E in a background of the susceptible inbred line Va35; to compare them to the QTL identified from Mp313E in a background of B73; and to test the stability of the QTL identified in Mp313E × Va35 in multiple environments by remapping the phenotypic tails of the Mp313E × Va35 mapping population in new locations. Twenty different QTL were found in this study, 11 of which were also found in different environments using the phenotypic tail subset mapping population, and five of which were likely the same as those reported in the Mp313E × B73 mapping population. This indicates that many of the QTL are stable over the environments and genetic backgrounds tested, which will make them more valuable in breeding efforts.

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Acknowledgments

The authors express our appreciation for the technical assistance of J.A. Haynes and L.T. Owens, critical reviews and suggestions by Drs. Xueyan Shan and C. Zila, and statistical advice from D. Boykin.

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Authors and Affiliations

  1. CIMMYT, Int. Apdo Postal 6-641, 06600, Mexico, DF, Mexico

    Martha C. Willcox

  2. Division of Plant Sciences, University of Missouri-Columbia, 1-31 Agriculture Bldg., Columbia, MO, 65211, USA

    Georgia L. Davis

  3. USDA ARS CHPRRU, P.O. Box 9555, Mississippi State, MS, 39762, USA

    Marilyn L. Warburton, Gary L. Windham & W. Paul Williams

  4. USDA ARS BCPRU, 59 Lee Rd., Stoneville, MS, 38776, USA

    Hamed K. Abbas

  5. Syngenta Seeds S.A.S., B.P 85127, 31151, Fenouillet Cedex, France

    Javier Betrán

  6. USDA ARS PSRU, Box 7620, Raleigh, NC, 27695, USA

    James B. Holland

Authors
  1. Martha C. Willcox
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  2. Georgia L. Davis
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  3. Marilyn L. Warburton
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  4. Gary L. Windham
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  5. Hamed K. Abbas
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  6. Javier Betrán
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  7. James B. Holland
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  8. W. Paul Williams
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Corresponding author

Correspondence to Marilyn L. Warburton.

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Willcox, M.C., Davis, G.L., Warburton, M.L. et al. Confirming quantitative trait loci for aflatoxin resistance from Mp313E in different genetic backgrounds. Mol Breeding 32, 15–26 (2013). https://doi.org/10.1007/s11032-012-9821-9

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  • DOI: https://doi.org/10.1007/s11032-012-9821-9

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