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Development and validation of a breeder-friendly KASPar marker for wheat leaf rust resistance locus Lr21

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Abstract

Development and utilization of genetic markers play a pivotal role in marker-assisted breeding of wheat cultivars with pyramids of disease resistance genes. The objective of this study was to develop a closed-tube, gel-free assay for high-throughput genotyping of leaf rust resistance locus Lr21. Polymorphism identified from re-sequencing of a 2.4-kb fragment covering the functional region of the Lr21 gene from the second to the fourth indels was targeted for assay development. The generated sequence data revealed the 88- or 105-bp indel in the first intron of the Lr21 gene in the selected resistant cultivars compared to susceptible US spring and winter wheat cultivars. Allele-specific primers for a KASPar assay were designed around the junction of the indel at position 1,346 bp. The marker was tested on a panel of 384 US wheat lines and found to be effective in differentiating resistant and susceptible genotypes.

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References

  • Chen W, Mingus J, Mammadov J, Backlund JE, Greene T, Thompson S, Kumpatla S (2010) KASPar: a simple and cost-effective system for SNP genotyping. In: Proceedings of plant and animal genomes XVIII conference. San Diego, p 94

  • Fu YB, Peterson GW, McCallum BD, Huang L (2010) Population based resequencing analysis of improved wheat germplasm at wheat leaf rust resistance locus Lr21. Theor Appl Genet 121:271–281

    Article  PubMed  CAS  Google Scholar 

  • Huang L, Brooks SA, Li W, Fellers J, Trick HN, Gill B (2003) Map based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of breed wheat. Genetics 164:655–664

    PubMed  CAS  Google Scholar 

  • Kolmer JA, Anderson JA (2011) First detection in North America of virulence in wheat leaf rust (Puccinia triticina) to seedling plants of wheat with Lr21. Plant Dis 95:1032

    Article  Google Scholar 

  • Kumpatla SP, Buyyarapu R, Abdurakhmonov IY, Mammadov JA (2012) Genomics-assisted plant breeding in the 21st century: technological advances and progress. In: Abdurakhmonov IY (ed) Plant breeding, pp 131–183, InTech publishers http://www.intechopen.com/books/plant-breeding

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Acknowledgments

The contribution of Mr. Jared Smith and Mrs. Sharon Williamson (USDA-ARS Eastern Regional Small Grains Genotyping, Laboratory) in designing KASPar assays and assay setup is acknowledged with thanks. This work was supported in part by funds provided by the USDA T-CAP AFRI grant number 2011-68002-30029.

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Authors and Affiliations

  1. Department of Crop Science, North Carolina State University, Raleigh, NC, 27695, USA

    Kumari Neelam & Gina Brown-Guedira

  2. USDA-ARS Plant Science Research, Raleigh, NC, 27695, USA

    Gina Brown-Guedira

  3. Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717, USA

    Li Huang

Authors
  1. Kumari Neelam
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  2. Gina Brown-Guedira
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  3. Li Huang
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Corresponding author

Correspondence to Gina Brown-Guedira.

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Neelam, K., Brown-Guedira, G. & Huang, L. Development and validation of a breeder-friendly KASPar marker for wheat leaf rust resistance locus Lr21 . Mol Breeding 31, 233–237 (2013). https://doi.org/10.1007/s11032-012-9773-0

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  • DOI: https://doi.org/10.1007/s11032-012-9773-0

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