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Discovery and use of single nucleotide polymorphic (SNP) markers in Jatropha curcas L.

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Abstract

Various programs for genetic improvement in oil yield of the biofuel plant Jatropha curcas L. are currently in progress worldwide. In order to develop strategies for genetic improvement, it is important to estimate the degree of diversity at the genetic level among various genotypes of J. curcas. High-throughput sequencing of complexity-reduced nuclear genomic DNA of J. curcas coupled with computational analysis discovered 2,482 informative single nucleotide polymorphisms (SNPs). Genotyping of selective SNPs among 148 global collections of J. curcas lines and further diversity analysis through NTSYS-pc, DARwin and Structure 2.0 software revealed that a narrow level of genetic diversity existed among the indigenous genotypes as compared to the exotic genotypes of J. curcas. The level of marker informativeness along with distance-based and Bayesian clustering revealed grouping of the accession from Togo (Africa) with various Indian accessions at K = 4 and K = 5 values (where K represents the number of populations). The diverse accessions identified in the study will be of further use in genetic improvement of J. curcas through quantitative trait loci and association mapping.

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Acknowledgments

This work was supported under a supra-institutional programme of the Council of Scientific and Industrial Research (CSIR), Government of India. The Director, NBRI, is acknowledged for providing basic infrastructure facilities for carrying out the work.

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Authors and Affiliations

  1. Plant Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, Uttar Pradesh, India

    Priya Gupta, Asif Idris, Mehar Hasan Asif, Hemant Kumar Yadav, Chandra Sekhar Mohanty & Samir Vishwanath Sawant

  2. National Agri-food Biotechnology Institute, C-127, Industrial Area, SAS Nagar, Phase 8, Mohali, 160 071, Punjab, India

    Shrikant Mantri, Joy Kumar Roy & Rakesh Tuli

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  1. Priya Gupta
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  2. Asif Idris
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Correspondence to Chandra Sekhar Mohanty.

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Gupta, P., Idris, A., Mantri, S. et al. Discovery and use of single nucleotide polymorphic (SNP) markers in Jatropha curcas L.. Mol Breeding 30, 1325–1335 (2012). https://doi.org/10.1007/s11032-012-9719-6

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  • DOI: https://doi.org/10.1007/s11032-012-9719-6

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