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Molecular and cytological characterization of introgression lines in yellow seed derived from somatic hybrids between Brassica napus and Sinapis alba

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Abstract

Polymerase chain reaction and genomic in situ hybridization techniques were conducted to demonstrate the genomic introgressions in somatic hybrid progenies between Brassica napus and Sinapis alba. With minisatellite core sequence 33.6 as primer, an S. alba-specific band (288 bp) was amplified in yellow seed progenies. No hybridization signals were found using the genomic DNA of S. alba as probe. In addition, degenerate primers were used for the detection of related genes based on the genes related to flavonoid biosynthesis of the model plant Arabidopsis thaliana. Sequencing results show that flavonoid pathway genes are highly conserved in A. thaliana, S. alba and B. napus, and present subtle differences because of genetic evolution. A specific band (1,672 bp) consistent with S. alba was characterized in the yellow seed lines. This study demonstrates that the new yellow seed germplasms, derived from backcrossed and self-crossed progenies of B. napus–S. alba hybrids, are stable and homozygous introgression lines with yellow seed color, and differ from existing yellow seed materials.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (30971812, 30671166) and the Natural Science Foundation of Jiangsu Province (BRA2010141, BK2009174).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China

    Aimin Li, Jinjin Jiang & Youping Wang

  2. Jiangsu Institute of Agricultural Science in the Lixiahe District, 225009, Yangzhou, China

    Aimin Li & Yongtai Zhang

  3. Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Rod J. Snowdon

  4. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, 225009, Yangzhou, China

    Guohua Liang

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  1. Aimin Li
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  2. Jinjin Jiang
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  3. Yongtai Zhang
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  4. Rod J. Snowdon
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  5. Guohua Liang
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  6. Youping Wang
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Correspondence to Youping Wang.

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Aimin Li and Jinjin Jiang have contributed equally to this work.

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Li, A., Jiang, J., Zhang, Y. et al. Molecular and cytological characterization of introgression lines in yellow seed derived from somatic hybrids between Brassica napus and Sinapis alba . Mol Breeding 29, 209–219 (2012). https://doi.org/10.1007/s11032-010-9540-z

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  • DOI: https://doi.org/10.1007/s11032-010-9540-z

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