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Fine mapping of Sta1, a quantitative trait locus determining stele transversal area, on rice chromosome 9

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Abstract

The stele (root vascular cylinder) in plants plays an important role in the transport of water and nutrients from the root to the shoot. A quantitative trait locus (QTL) on rice chromosome 9 that controls stele transversal area (STA) was previously detected in an F3 mapping population derived from a cross between the lowland cultivar ‘IR64’, with a small STA, and the upland cultivar ‘Kinandang Patong’, with a large STA. To identify the gene(s) underlying this QTL, we undertook fine mapping of the locus. We screened eight plants from BC2F3 lines in which recombination occurred near the QTL. Progeny testing of BC2F4 plants was used to determine the genotype classes for the QTL in each BC2F3 line. Accordingly, the STA QTL Sta1 (Stele Transversal Area 1) was mapped between the InDel markers ID07_12 and ID07_14. A candidate genomic region for Sta1 was defined more precisely between markers RM566 and RM24334, which delimit a 359-kb interval in the reference cultivar ‘Nipponbare’. A line homozygous for the ‘Kinandang Patong’ allele of Sta1 had an STA approximately 28.4% larger than that of ‘IR64’. However, Sta1 did not influence maximum or total root length, suggesting that this QTL specifically controls STA.

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Acknowledgments

We thank the staff of the technical support section of NIAS for their field management and experimental support. This work was supported by grants from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Agri-Genome Project QT-2001, Genomics for Agricultural Innovation QTL-4003).

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  1. Kazutoshi Okuno

    Present address: University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

Authors and Affiliations

  1. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Yusaku Uga & Masahiro Yano

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  1. Yusaku Uga
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  2. Kazutoshi Okuno
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  3. Masahiro Yano
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Correspondence to Yusaku Uga.

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Uga, Y., Okuno, K. & Yano, M. Fine mapping of Sta1, a quantitative trait locus determining stele transversal area, on rice chromosome 9. Mol Breeding 26, 533–538 (2010). https://doi.org/10.1007/s11032-010-9450-0

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