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Elucidation of origin of the present day hybrid banana cultivars using the 5′ETS rDNA sequence information

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Abstract

In the present study, our intention was to elucidate the genetic relation of M. acuminata subspecies and analyse the diversity of the M. balbisiana gene-pool using nuclear ribosomal gene loci based marker system. Additionally the obtained information allowed elucidating the structure and ancestry of the nuclear genomes of diploid and triploid cultivars. By establishing the nucleotide sequence of the rDNA locus for M. acuminata and partially for M. balbisiana and their comparative analysis revealed that the 5′ETS region was the most divergent between acuminata and balbisiana genomes. Based on the SNP sites identified in this region a PCR based system was built, which revealed four gene-pools among M. acuminata wild types, while M. balbisiana showed no sequence divergence. The developed markers proved to be a powerful tool in the identification of the acuminata component of diploid and triploid hybrid cultivars and discovery of unexpected genotypes.

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Acknowledgments

The present work was funded by Bioversity International (formerly International Network for Improvement of Banana and Plantain (INIBAP)). The authors are grateful to Prof. Benchamas Silayoi and Assoc. Prof. Dr. Somsak Apisitwanich for guidance and technical advice in bananas and Assoc. Prof. Dr. Jaroslav Doležel for kindly providing us with DNA from some banana wild type species.

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  1. Biogenetics/Natural resources, Austrian Research Centers GmbH—ARC, 2444, Seibersdorf, Austria

    Ratri Boonruangrod, Silvia Fluch & Kornel Burg

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  1. Ratri Boonruangrod
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  2. Silvia Fluch
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  3. Kornel Burg
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Correspondence to Ratri Boonruangrod.

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Boonruangrod, R., Fluch, S. & Burg, K. Elucidation of origin of the present day hybrid banana cultivars using the 5′ETS rDNA sequence information. Mol Breeding 24, 77–91 (2009). https://doi.org/10.1007/s11032-009-9273-z

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  • DOI: https://doi.org/10.1007/s11032-009-9273-z

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