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Development, identification, and validation of markers for marker-assisted selection against the Stagonospora nodorum toxin sensitivity genes Tsn1 and Snn2 in wheat

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Abstract

The wheat-Stagonospora nodorum pathosystem involves a number of pathogen-produced host-selective toxins that interact with host genes in an inverse gene-for-gene manner to cause disease. The wheat intervarietal recombinant inbred population derived from BR34 and Grandin (BG population) segregates for the toxin sensitivity genes Tsn1, Snn2, and Snn3, which confer sensitivity to the toxins ToxA, SnTox2, and SnTox3, respectively. Here, we report the addition of 141 molecular markers to the BG population linkage maps, the identification and/or development of markers tightly linked to Tsn1 and Snn2, and the validation of the markers using a set of diverse wheat accessions. The BG population maps now contain 787 markers, and new simple sequence repeat (SSR) markers closely linked to Snn2 on chromosome arm 2DS were identified. In an effort to target more markers to the Snn2 locus, STS markers were developed from 2DS bin-mapped ESTs resulting in the development and mapping of 36 markers mostly to the short arms of group 2 chromosomes. Together, SSR and EST-STS markers delineated Snn2 to a 4.0 cM interval. SSRs developed in related work for Tsn1 were mapped in the BG population and delineated the gene to a 1.0 cM interval. Evaluation of the markers for Tsn1 and Snn2 in a diverse set of wheat genotypes validated their utility for marker-assisted selection, which is particularly efficient for removing toxin sensitivity alleles from elite germplasm and varieties.

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Acknowledgements

This work was supported by USDA-ARS CRIS project 5442-22000-030-00D and by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, Grant Number 2003-35300-13109 to J.D.F.

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Authors and Affiliations

  1. Department of Plant Sciences, Loftsgard Hall, North Dakota State University, Fargo, ND, 58105, USA

    Zengcui Zhang

  2. Northern Crop Science Laboratory, USDA-ARS Cereal Crops Research Unit, Red River Valley Agricultural Research Center, 1307 18th Street North, Fargo, ND, 58105, USA

    Timothy L. Friesen, Kristin J. Simons, Steven S. Xu & Justin D. Faris

Authors
  1. Zengcui Zhang
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  2. Timothy L. Friesen
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  3. Kristin J. Simons
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  4. Steven S. Xu
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  5. Justin D. Faris
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Corresponding author

Correspondence to Justin D. Faris.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Zhang, Z., Friesen, T.L., Simons, K.J. et al. Development, identification, and validation of markers for marker-assisted selection against the Stagonospora nodorum toxin sensitivity genes Tsn1 and Snn2 in wheat. Mol Breeding 23, 35–49 (2009). https://doi.org/10.1007/s11032-008-9211-5

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  • DOI: https://doi.org/10.1007/s11032-008-9211-5

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