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Detection of Fusarium head blight resistance QTLs using five populations of top-cross progeny derived from two-row × two-row crosses in barley

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Abstract

Fusarium head blight (FHB) resistance was evaluated in five recombinant inbred (RI) populations. The RI populations consisted of top-cross progeny derived from a diallel set of crosses. Each of five two-row barley lines differing in response to FHB were crossed with ‘Harbin 2-row’. FHB severity was scored on an 11-point scale, where resistant = 0 and susceptible = 10, based on the ‘cut-spike test’. Disease data were obtained for each population for 2 or 3 years. Linkage maps comprised of expressed sequence tag (EST) markers were developed for each population and used for quantitative trait locus (QTL) detection. Thirty two QTLs were detected using all data sets (individual populations and years). Thirteen QTLs were detected using averages across years; 10 of these were consistent across the individual year and average data sets. These QTLs clustered at 14 regions, with clusters on all chromosomes. At 11 of these clusters, Harbin 2-row contributed FHB resistance alleles. No QTLs were detected near the row type (vrs1) locus in any of the five RI populations, suggesting that the FHB resistance QTL in this region reported in two-row × six-row crosses may be pleiotropic effect of vrs1. QTL were coincident with the flowering type locus (cly1/Cly2) on chromosome 2H in every population. Some QTL × QTL interactions were significant, but these were smaller than QTL main effects. Considering the pleiotropic effect of spike morphology on FHB resistance, future FHB resistance mapping efforts in barley should focus on cross combinations in which alleles at vrs1 are not segregating.

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Abbreviations

FHB:

Fusarium head blight

RI:

Recombinant inbred

EST:

Expressed sequence tag

QTL:

Quantitative trait locus

SNP:

Single nucleotide polymorphism

AFLP:

Amplified fragment length polymorphism

SSR:

Simple sequence repeat

STS:

Sequence tagged site

CAPS:

Cleaved amplified polymorphic sequence

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Acknowledgements

We would like to thank Dr. Patrick M. Hayes for his critical reading of the manuscript, Ms. N. Nankaku and Y. Motoi, Res. Inst. Biores., Okayama Univ., for their technical assistance.

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Authors and Affiliations

  1. Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, 710-0046, Japan

    Kazuhiro Sato, Kiyosumi Hori & Kazuyoshi Takeda

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  1. Kazuhiro Sato
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  2. Kiyosumi Hori
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  3. Kazuyoshi Takeda
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Correspondence to Kazuhiro Sato.

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Sato, K., Hori, K. & Takeda, K. Detection of Fusarium head blight resistance QTLs using five populations of top-cross progeny derived from two-row × two-row crosses in barley. Mol Breeding 22, 517–526 (2008). https://doi.org/10.1007/s11032-008-9195-1

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