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Markers associated with a QTL for grain yield in wheat under drought

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Abstract

Drought is a major abiotic stress that adversely affects wheat production in many regions of the world. The objective of this study was to identify quantitative trait loci (QTL) controlling grain yield and yield components under reduced moisture. A cross between common wheat cultivars ‘Dharwar Dry’ (drought tolerant) and ‘Sitta’ was the source of one hundred twenty-seven recombinant inbred lines evaluated for two-seasons in a field under differing soil moisture regimes in Ciudad Obregon, Sonora, Mexico. An SSR/EST-STS marker map was constructed and a grain yield QTL on the proximal region of chromosome 4AL was found to have a significant impact on performance under reduced moisture. This region was associated with QTL for grain yield, grain fill rate, spike density, grains m−2, biomass production, biomass production rate, and drought susceptibility index (DSI). Molecular markers associated with these traits explained 20, 33, 15, 23, 30, 26, and 41% of phenotypic variation, respectively on chromosome 4A. Microsatellite locus Xwmc89 was associated with all significant QTL covering a 7.7 centiMorgans (cM) region and generally explained the greatest proportion of phenotypic variation. The alleles associated with enhanced performance under drought stress were contributed by Dharwar Dry. Microsatellite marker wmc89 may be useful for marker assisted selection to enhance drought tolerance.

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Authors and Affiliations

  1. Department of Agronomy, Kansas State University, Manhattan, KS, 66502, USA

    F. M. Kirigwi, G. M. Paulsen & A. K. Fritz

  2. Department of Primary industries (DPI), DX Number: 216515, Private Bag 260, Horsham, VIC, 3401, Australia

    M. Van Ginkel

  3. USDA-ARS Eastern Regional Small Grains Genotyping Lab, 840 Main Campus Dr, Raleigh, NC, 27606, USA

    G. Brown-Guedira

  4. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66502, USA

    B. S. Gill

Authors
  1. F. M. Kirigwi
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  2. M. Van Ginkel
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  3. G. Brown-Guedira
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  4. B. S. Gill
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  5. G. M. Paulsen
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  6. A. K. Fritz
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Correspondence to F. M. Kirigwi.

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Kirigwi, F.M., Van Ginkel, M., Brown-Guedira, G. et al. Markers associated with a QTL for grain yield in wheat under drought. Mol Breeding 20, 401–413 (2007). https://doi.org/10.1007/s11032-007-9100-3

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  • DOI: https://doi.org/10.1007/s11032-007-9100-3

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