Abstract
Sequences annotated as aspartate aminotransferases (synonymous with glutamate oxaloacetate transaminases) in the SOL Genomics Network unigene database were used to design 10 pairs of PCR primers for genetic marker development. These primer pairs generated nine CAPS markers, two SCAR markers and one SSR marker, which were bin-mapped using a set of tomato introgression lines (IL) derived from Lycopersicon esculentum cv. M82 and Lycopersicon pennellii LA716. Based on their bin locations, these markers are largely dispersed throughout the tomato genome and appear to have tagged all four of the glutamate oxaloacetate transaminase (Got) isozyme marker genes placed on the classical genetic map of tomato. Orthologous relationships with Arabidopsis aspartate aminotransferase (Asp) genes suggest the existence of at least two additional functional Got genes in tomato that have also been tagged by these markers and likewise an additional functional Asp gene in Arabidopsis. The Got-2 isozyme marker has often been used for the marker-assisted breeding of the I-3 gene for Fusarium wilt resistance introgressed from L. pennellii LA716. The Got-2 CAPS marker that we have developed offers a facile PCR-based alternative to the isozyme marker for the marker-assisted breeding of I-3. However, all of the PCR-based markers we have developed have the potential to assist the breeding of linked traits introgressed from wild relatives of tomato.
References
Arabidopsis Genome Initiative. (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815
Bournival BL, Scott JW, Vallejos CE (1989) An isozyme marker for resistance to race 3 of Fusarium oxysporum f. sp. lycopersici in tomato. Theor Appl Genet 78:489–494
Eshed Y, Zamir D (1994) A genomic library of Lycopersicon pennellii in L. esculentum: a tool for fine mapping of genes. Euphytica 79:175–179
Gunther S, Postel W, Wiering H, Gorg A (1988) Acid phosphatase typing for breeding nematode-resistant tomatoes by isoelectric focusing with an ultranarrow immobilized pH gradient. Electrophoresis 9:618–620
Liepman AH, Olsen LJ (2004) Genomic analysis of aminotransferases in Arabidopsis thaliana. Crit Rev Plant Sci 23:73–89
Moore PP, Sink KC (1988) Characterization of a Lycopersicon esculentum × Solanum lycopersicoides somatic hybrid lacking a glutamate oxaloacetate transaminase isozyme. Plant Cell Tissue Organ Cult 13:39–47
Reumann S (2004) Specification of the peroxisome targeting signals type 1 and type 2 of plant peroxisomes by bioinformatics analyses. Plant Physiol 135:783–800
Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Acknowledgements
This project was funded by the Biotechnology Research Centre, Research School of Biological Sciences, The Australian National University and the Cooperative Research Centre for Tropical Plant Protection. The authors are grateful to the plant culture staff of the Research School of Biological Sciences for plant care.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, GP., Lim, G.T.T. & Jones, D.A. Development of PCR-based markers from the tomato glutamate oxaloacetate transaminase isozyme gene family as a means of revitalising old isozyme markers and recruiting new ones. Mol Breeding 19, 209–214 (2007). https://doi.org/10.1007/s11032-006-9037-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11032-006-9037-y