Abstract
Synthetic hexaploid wheats (SHWs) and synthetic backcross-derived lines (SBLs) obtained from them are novel sources of useful traits for broadening the diversity in breeding germplasm of hexaploid bread wheat (Triticum aestivum). Fifty-one EST-derived and 39 genomic-derived microsatellite markers (SSRs) covering the A, B, and D genomes were used to assess the genetic diversity present in 11 SHWs, their backcross derived families, and their durum and bread wheat parents and to test for the selective advantage of SHW alleles in SBL families after several generations of selection. The 90 SSR markers amplified 91 loci with 474 alleles across all genotypes. In many of the SHWs, novel alleles were observed which were stably inherited in the SBL families. Gene diversity, the average number of alleles per locus, cluster analysis, and principal coordinate analysis revealed a high level of genetic diversity in the Aegilops tauschii and durum parents of the SHWs, and also in the SBLs. In the latter, alleles from the SHW parent had a selective advantage for six SSR markers. This indicates that SHWs and SBLs are a valuable resource for broadening the genetic base of elite wheat breeding germplasm. Fingerprinting of SBLs and their corresponding SHW and bread wheat parents, and testing for selective advantage of SHWs alleles promises to be a useful method for detecting chromosomal regions of interest for bread wheat improvement.
Similar content being viewed by others
References
M.J. Christiansen S.B. Anderson R. Ortiz (2002) ArticleTitleDiversity changes in an intensively bread wheat germplasm during the 20th century Mol. Breed. 9 1–11
I.A. Del Blanco S. Rajaram W.E. Kronstad M.P. Reynolds (2000) ArticleTitlePhysiological performance of synthetic hexaploid wheatderived populations Crop Sci. 40 1257–1263
I.A. Del Blanco S. Rajaram W.E. Kronstad (2001) ArticleTitleAgronomic potential of synthetic hexaploid wheat-derived populations Crop Sci 41 670–676
K.M. Devos G.J. Bryan P. Stephenson M.D. Gale (1995) ArticleTitleApplication of two microsatellite sequences in wheat storage proteins as molecular markers Theor. Appl. Genet. 90 247–252
M. Diaby M.D. Casler (2003) ArticleTitleRAPD marker variation among smooth bromegrass cultivars Crop Sci. 43 1538–1547
S. Dreisigacker P. Zhang M.L. Warburton M. Ginkel Particlevan D. Hoisington M. Bohn A.E. Melchinger (2004) ArticleTitleSSR and pedigree analyses of genetic diversity among CIMMYT wheat lines targeted to different mega-environments Crop Sci. 44 381–388
Dufner J., Jensen U. and Schumacher E. 2002. Statistik mit SAS. B.G. Teubner, Stuttgart, Germany, pp. 212.
J. Dvorak M.C. Luo Z.L. Yang H.B. Zhang (1998) ArticleTitleThe structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat Theor. Appl. Genet. 97 657–670
I. Eujayl M. Sorrells M. Baum P Wolters W. Powell (2002) ArticleTitleIsolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat Theor. Appl. Genet. 104 399–407
I. Eujayl M. Sorrells M. Baum P Wolters W. Powell (2002) ArticleTitleIsolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat Theor. Appl. Genet. 104 399–407
M.M. Frisch M. Bohn A.E. Melchinger (2000) ArticleTitlePlabsim: Software for simulation of marker-assisted backcrossing J. of Heredity 91 86–87
N.N. Gororo H.A. Eagles R.F. Eastwood M.E. Nicolas R.G. Flood (2002) ArticleTitleUse of Triticum tauschii to improve yield of wheat in low-yielding environments Euphytica 123 IssueID2 241–254 Occurrence Handle10.1023/A:1014910000128
J.C. Gower (1966) ArticleTitleSome distance properties of latent root and in multivariate analysis Biometricka 53 325–388
M. Heckenberg A.E. Melchinger J.S. Ziegle L.K. Joe J.D. Hauser M. Hutton M. Bohn (2002) ArticleTitleVariation of DNA fingerprints among accessions within maize inbred lines with regard to the identification of essentially derived varieties. I. Genetic and technical sources of variation in SSR data Mol. Breed. 10 181–191
D. Hoisington M. Khairallah T. Reeves J.M. Ribaut B. Skovmand S. Taba M. Warburton (1999) ArticleTitlePlant genetic resources: what can they contribute toward increased crop productivity?Proc Natl. Acid. Sci. USA 96 5937–5943
R. Ihaka R. Gentleman (1996) ArticleTitleA language for data analysis and graphics J. of Computational and Graphical Statistics 5 299–314
G.H.J. Kema W. Lange C.H. Silfhout (1995) ArticleTitleDifferential suppression of stripe rust resistance in synthetic wheat hexaploids derived from Triticum turgidum subsp. dicoccoides and Aegilops squarrosa Phytopathology 85 425–429
Lage J., Skovmand B. and Andersen S.B. 2001. Field evaluation of Russian wheat aphid (Homoptera: Aphididae) resistance in Triticum dicoccum derived synthetic hexaploid wheats. Abstract, Annual meeting, ESA, San Diego, USA, December 2001.
J. Lage B. Skovmand S.B. Andersen (2002) ArticleTitleExpression and suppression of resistance to greenbug (Homoptera: Aphididae) in synthetic hexaploid wheats derived from Triticum dicoccum x Aegilops tauschii crosses J. of Economic Entomology 96 202–206
T. Lelley M. Stachel H. Grausgruber J. Vollmann (2000) ArticleTitleAnalysis of relationships between Aegilops tauschii and the D genome of wheat utilizing microsatellites Genome 43 661–668
B. Liu J.M. Vega G. Segal S. Abbo M. Rodova M. Feldman (1998a) ArticleTitleRapid genomic changes in newly synthesized polyploids of Triticum and Aegilops. I. Changes in low-copy noncoding DNA sequences Genome 41 272–277
B. Liu J.M. Vega M. Feldman (1998) ArticleTitleRapid genomic changes in newly synthesized polyploids of Triticum and Aegilops. II.Changes in low-copy coding DNA sequences Genome 41 535–542
H. Ma R.P. Singh A. Mujeeb-Kazi (1995) ArticleTitleResistance to stripe rust in Triticum turgidum, A. tauschii and their synthetic hexaploids Euphytica 82 117–124
A. Mujeeb-Kazi V. Rosas S. Roldan (1996) ArticleTitleConservation of the genetic variation of Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa auct. non L.) in synthetic hexaploid wheats (T. turgidum L. x A. tauschii; 2n = 6x =42, AABBDD) and its potential utilization for wheat improvement Genet. Res. Crop. Evol. 43 129–134
A. Mujeeb-Kazi G. Fuentes-Davila R.L. Villareal A. Cortes V. Roasas R. Delgado (2001a) ArticleTitleRegistration of 10 synthetic hexaploid wheat and six bread wheat germplasms resistant to karnal bunt Crop Sci. 41 1652–1653
A. Mujeeb-Kazi L.I. Gilchrist R.L. Villareal R. Delgado (2001b) ArticleTitleRegistration of ten wheat germplasms resistant to Septoria tritici leaf blotch Crop Sci 40 590–591
M. Nei (1987) Molecular evolutionary genetics Columbia University Press New York, USA 164
M. Nei (1987) Molecular evolutionary genetics Columbia University Press New York, USA 164
E. Pestsova V. Korzun N.P. Goncharov K. Hammer M.W. Ganal M.S. RÖ (2000) ArticleTitleMicrosatellite analysis of Aegilops tauschii germplasm Theor. Appl. Genet. 101 100–106
Rajaram S. 2001. The human right to food and livelihoods: the role of global wheat research. Presented at the national seminar, building on past achievements to advance the future direction of Australian agriculture, ASTE Crawford Fund and Australian Institute of Agricultural Science and Technology, 2001, University House, Canberra, Australia.
J.R. Russell R.P. Ellis W.T.B. Thomas R. Waugh J. Provan A. Booth J. Fuller P. Lawrence G. Young W. Powell (2000) ArticleTitleA retrospective analysis of spring barley germplasm development from ‘foundation genotypes’ to currently successful cultivars Mol. Breed. 6 553–568
M.A. Saghai-Maroof K. Soliman R.A. Jorgensen R.W. Allard (1984) ArticleTitleRibosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics Proc. Natl. Acad. Sci. USA 81 8014–8018
F. Salamini H. Özkan A. Brandolini R. Schäfer-Pregl W. Martin (2002) ArticleTitleGenetics and geography of wild cereal domestication in the Near East Nature Rev. Genet. 3 429–441
Sasakuma T., Ogihara Y. and Tsujimoto H. 1995. Genome rearrangement of repetitive sequence in processes of hybridization and amphiploidization in Triticinae.. In: Li Z.S. and Xin Z.Y. (eds), Proc. of the 8th Intl. Wheat Genet. Symp. Held at Beijing, China, 20-25 July 1993. China Agricultural Scientech Press, Beijing, China, pp. 563–566.
E.M. Sharon S. Kresovich C.A. Jester C.J. Hernandez A.K. Szewc-McFadden (1997) ArticleTitleApplication of multiplex PCR and fluorescence-based, semi-automated allele sizing technology for genotyping plant genetic resources Crop Sci. 37 617–624
K.M. Song P. Lu K.L. Tang T.C. Osborn (1995) ArticleTitleRapid genome changes in synthetic polyploids of Brassica and its implications for polyploid evolution Proc. Natl. Acad. Sci. USA 92 7719–7723
S.D. Tanksley S.R. McCouch (1997) ArticleTitleSeed banks and molecular maps: Unblocking genetic potential from the wild Science 277 1063–1066 Occurrence Handle10.1126/science.277.5329.1063 Occurrence Handle1:CAS:528:DyaK2sXlsFSisrw%3D Occurrence Handle9262467
R.L. Villareal A. Mujeeb-Kazi E. Del Toto J. Crossa S. Rajaram (1994a) ArticleTitleAgronomic variability in selected Triticum turgidum x A. tauschii synthetic hexaploid wheats J. Agron and Crop Sci. 173 307–317
R.L. Villareal A. Mujeeb-Kazi S. Rajaram E. Del Toto (1994) ArticleTitleMorphological variability in some synthetic hexaploid wheats derived from Triticum turgidum x A. tauschii J. Genet. and Breed. 48 7–16
R.L. Villareal E. Del Toro A. Mujeeb-Kazi S. Rajaram (1995) ArticleTitleThe 1BL/1RS chromosome translocation effect on yield characteristics in a Triticum aestivum L. cross Plant Breed. 114 497–500
R.L. Villareal K. Sayre O. Banuelos A. Mujeeb-Kazi (2001) ArticleTitleRegistration of four synthetic hexaploid wheat (Triticum turgidum/Aegilops tauschii) germplasm lines tolerant to waterlogging Crop Sci. 41 274
Wright S. 1978. Evolution and the genetics of populations. University Chicago Press, Chicago III, USA.
D. Zohary J.R. Harlan A. Vardi (1969) ArticleTitleThe wild diploid progenitors of wheat and their breeding value Euphytica 18 58–65
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, P., Dreisigacker, S., Melchinger, A.E. et al. Quantifying novel sequence variation and selective advantage in synthetic hexaploid wheats and their backcross-derived lines using SSR markers. Mol Breeding 15, 1–10 (2005). https://doi.org/10.1007/s11032-004-1167-5
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11032-004-1167-5