Abstract
Chorionic villi are finger like projections, which surrounds the embryonic sac in early pregnancy and will later form the placenta. The outer layer of the chorionic villi consists of trophoblast cells. These cells are actively dividing and can be used for direct preparation or short-term culture for prenatal diagnosis of chromosomal abnormalities. We herein describe a 100% reproducible modified technique for one day direct culturing (direct prepara-tion) of trophoblast cells. Direct preparation of chorionic villi provides the physician with additional diagnostic information during prenatal diagnosis and genetic counseling. Direct preparation also eliminates the risk of maternal cell contamination. This method was 100% reproducible provided all the precautions mentioned herewith were taken into consideration and can be completed in 45 minutes upon arrival of the samples in the laboratories.
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References
Ledbetter DR, Martin AO, Verlinsky Y, Pergament E, Jackson L, Yang-Feng T, Schonberg SA, Gilbert F, Zachary JM, Barr M et al. (1990). Cytogenetic results of chorionic villus sampling: high success rate and diagnostic accuracy in the United States collaborative study. Am J Obstet Gynecol 162: 495–501
Phillips OP, Tharapel AT, Lerner JL, Park VM, Wachtel SS, Shulman LP (1996). Risk of fetal mosaicism when placental mosaicism is diagnosed by chorionic villus sampling. Am J Obstet Gynecol 174: 850–855
Ledbetter DH, Zachary JM, Simpson JL, Golbus MS, Pergament E, Jackson L, Mahoney MJ, Desnick RJ, Schulman J, Copeland KL et al. (1992). Cytogenetic results from a US collaborative study on CVS. Prenatal Diagn 12: 317–345
Simoni G, Brambati B, Danesino C, Rossella F, Terzoli G1, Ferrari M, Fraccaro M. (1983). Efficient direct chromosome analyses and enzyme determinations from Chorionic villi samples in the first trimester of preg-nancy. Hum Genet 63: 349–357
Sambrook J, Fritsch EF, Maniatis T (1989). Molecular cloning a laboratory Manual, Second Edition. Cold Spring Harbor Laboratory Press, B.12
Barch MI, Lawce HI, Arsham MS (1991). Peripheral blood culture. In: Barch MI (ed), The ACT Cytogenetics Laboratory Manual, Second Edition. New York: Raven Press, pp. 17–30
Dutrillaux B, Viegas-Pequinot E (1981). High resolution R and G-banding on the same preparation. Hum Genet 57: 93–95
Miller W, Marks B (1982). Culture of abortus material. Karyogram 8: 44–46
Danihel L, Gomolcal P, Korbel M, Pruzinec J, Vojtassak J, Janik P, Babal P (2002). Expression of proliferation and apoptotic markers in human placenta during pregnancy. Acta Histochem 104: 335–338
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Shetty, S., Gogate, A., Gogate, S. et al. A reproducible modified method for direct preparation of chorionic villi cytogenetic analysis. Methods Cell Sci 25, 149–154 (2004). https://doi.org/10.1007/s11022-004-6830-z
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DOI: https://doi.org/10.1007/s11022-004-6830-z