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Evaluation of the Phylogenetic Diversity of Prokaryotic Microorganisms in Sphagnum Peat Bogs by Means of Fluorescence In Situ Hybridization (FISH)

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Abstract

The microbial population of sphagnum peat bogs of northern Russia was analyzed with respect to the presence and cell numbers of representatives of particular phylogenetic groups of prokaryotes by means of in situ hybridization with fluorescently labeled group-specific rRNA-targeted oligonucleotide probes with broad detection spectra. The total number of cells that hybridized with universal Archaea- and Bacteria-specific probes varied, in peat samples of different bogs, from 45 to 83% of the number of cells revealed by DAPI staining. Down the bog profiles, the total number of prokaryotes and the fraction of archaea among them increased. Application of a set of oligonucleotide probes showed that the number of microorganisms belonging to such phylogenetic lineages of the domain Bacteria as the phyla Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, and Planctomycetes constituted, in total, 14.0–26.5% of the number of eubacteria detected in the samples. Among the bacteria identified in the peat samples, the most abundant were representatives of the classes Alphaproteobacteria and Betaproteobacteria and the phyla Acidobacteria, Bacteroidetes, and Actinobacteria.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    T. A. Pankratov, S. E. Belova & S. N. Dedysh

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  1. T. A. Pankratov
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  2. S. E. Belova
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  3. S. N. Dedysh
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Correspondence to S. N. Dedysh.

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Translated from Mikrobiologiya, Vol. 74, No. 6, 2005, pp. 831–837.

Original Russian Text Copyright © 2005 by Pankratov, Belova, Dedysh.

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Pankratov, T.A., Belova, S.E. & Dedysh, S.N. Evaluation of the Phylogenetic Diversity of Prokaryotic Microorganisms in Sphagnum Peat Bogs by Means of Fluorescence In Situ Hybridization (FISH). Microbiology 74, 722–728 (2005). https://doi.org/10.1007/s11021-005-0130-8

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  • DOI: https://doi.org/10.1007/s11021-005-0130-8

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