Skip to main content
SpringerLink
Log in

Analysis of DNA, lipopolysaccharide structure, and some cultural and morphological properties in closely related strains of Azospirillum brasilense

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

We studied closely related Azospirillum brasilense strains Sp7 and Cd. For probing of their genomes, the fragments of 85 MDa (p85) and 120 MDa (p120) from A. brasilense Sp245 plasmids were hybridized with 115-MDa (p115) and 90-MDa (p90) plasmids of strain Sp7, respectively. Strain Cd was found to lose the 115-MDa plasmid and one of the two EcoRI restriction fragments of the total DNA (localized within p115 and the chromosome) that was homologous to an EcoRI-generated p85 fragment of 2.4 kb. On the contrary, in the total DNA of strain Sp7-S, in spite of the previously established disappearance of the 115-MDa replicon, two fragments homologous to p85 were revealed, as with strain Sp7. It is suggested that the Sp7-S genome contains the total p115 DNA or at least a certain part of it. Strains Sp7 and Cd were found to differ in size and morphology of colonies on solid and semisolid media, in the levels of resistance to the cation surfactant cetavlon, and in the antigen structure of lipopolysaccharides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Katsy, E.I., The Properties and Functions of Plasmids in the Plant-associated Bacteria of the Genus Azospirillum, Usp. Sovrem. Biol., 2002, vol. 122, no.4, pp. 353–364.

    CAS  Google Scholar 

  2. Katzy, E.I., Matora, L.Yu., Serebrennikova, O.B., and Scheludko, A.V., Involvement of a 120-MDa Plasmid of Azospirillum brasilense Sp245 in Production of Lipopolysaccharides, Plasmid, 1998, vol. 40, no.1, pp. 73–83.

    Article  CAS  PubMed  Google Scholar 

  3. Vanbleu, E., Marchal, K., Lambrecht, M., Mathys, J., and Vanderleyden, J., Annotation of the pRHICO Plasmid of Azospirillum brasilense Reveals Its Role in Determining the Outer Surface Composition, FEMS Microbiol. Letts., 2004, vol. 232, no.2, pp. 165–172.

    Article  CAS  Google Scholar 

  4. Katsy, E.I., Borisov, I.V., Petrova, L.P., and Matora, L.Yu., The Use of the 85-and 120-MDa Plasmid Fragments from Azospirillum brasilense Sp245 to Study Plasmid Rearrangements in This Bacterium and to Search for New Homologous Plasmid Sequences in Azospirillum brasilense Sp7, Genetika, 2002, vol. 38, no.2, pp. 182–189.

    CAS  PubMed  Google Scholar 

  5. Matveev, V.Yu., Petrova, L.P., Zhuravleva, E.A., and Panasenko, V.I., Dissociation in Azospirillum brasilense Sp7 Cultures, Mol. Genet. Mikrobiol. Virusol., 1987, no. 8, pp. 16–18.

  6. Matora, L.Yu., Serebrennikova, O.B., Petrova, L.P., Burygin, G.L., and Shchegolev, S.Yu., Atypical R-S Dissociation in Azospirillum brasilense, Mikrobiologiya, 2003, vol. 72, no.1, pp. 60–63.

    Google Scholar 

  7. Katsy, E.I., Borisov, I.V., Petrova, L.P., and Shelud’ko, A.V., Genetic and Phenotypic Variability in the Populations of Azospirillum brasilense, Molecular Plant-Microbe Interactions: New Bridges between Past and Future, Abstracts of the XIth International Congress on Molecular Plant-Microbe Interactions, St. Petersburg, 2003, p. 311.

  8. Tarrand, J.X., Krieg, N.E., and Döbereiner, J., A Taxonomic Study of the Spirillum lipoferum Group, with Descriptions of a New Genus, Azospirillum gen. nov. and Two Species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov., Can. J. Microbiol., 1978, vol. 24, no.8, pp. 967–980.

    Google Scholar 

  9. Eskew, D.L., Focht, D.D., and Ting, I.P., Nitrogen Fixation, Denitrification, and Pleomorphic Growth in a Highly Pigmented Spirillum lipoferum, Appl. Environ. Microbiol., 1977, vol. 34, no.5, pp. 582–585.

    CAS  PubMed  Google Scholar 

  10. De Mot, R. and Vanderleyden, J., Application of Two-Dimensional Protein Analysis for Strain Fingerprinting and Mutant Analysis of Azospirillum Species, Can. J. Microbiol., 1989, vol. 35, no.10, pp. 960–967.

    Google Scholar 

  11. Gündisch, C., Baur, M., Kirchhof, G., Bode, W., and Hartmann, A., Characterization of Azospirillum Strains by RFLP and Pulsed-Field Gel Electrophoresis, Microbiol. Releases, 1993, vol. 2, no.1, pp. 41–45.

    Google Scholar 

  12. Giovanetti, L., Ventura, S., Bazzicalupo, M., Fani, R., and Materassi, R., DNA Restriction Fingerprint Analysis of the Soil Bacterium Azospirillum, J. Gen. Microbiol., 1990, vol. 136, no.6, pp. 1161–1166.

    PubMed  Google Scholar 

  13. Kirchhof, G., Schloter, M., Assmus, B., and Hartmann, A., Molecular Microbial Ecology Approaches Applied to Diazotrophs Associated with Non-Legumes, Soil Biol. Biochem., 1997, vol. 29, nos.5–6, pp. 853–862.

    Article  Google Scholar 

  14. Baldani, V.L.D., Baldani, J.I., and Döbereiner, J., Effects of Azospirillum Inoculation on Root Infection and Nitrogen Incorporation in Wheat, Can. J. Microbiol., 1983, vol. 29, no.8, pp. 924–929.

    Google Scholar 

  15. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor: Cold Spring Harbor Lab., 1989.

    Google Scholar 

  16. Döbereiner, J. and Day, J.M., Associative Symbiosis in Tropical Grasses: Characterization of Microorganisms and Dinitrogen Fixing Sites, Int. Symp. on Nitrogen Fixation: Interdisciplinary Discussion, Newton, W.E. and Nijmans, C.J., Eds., Wash. State Univ. Press, 1976, vol. 2, pp. 518–538.

  17. Matora, L.Yu., Shvartsburd, B.I., and Shchegolev, S.Yu., Immunochemical Analysis of O-Specific Polysaccharides from the Soil Nitrogen-fixing Bacterium Azospirillum brasilense, Mikrobiologiya, 1998, vol. 67, no.6, pp. 815–820.

    Google Scholar 

  18. Dubois, M., Gilles, K.A., Hamilton, J.K., Roberts, P.A., and Smith, F., Colorimetric Method for Determination of Sugar and Related Substances, Anal. Biochem., 1956, vol. 72, no.3, pp. 350–356.

    Google Scholar 

  19. Eckhardt, T., A Rapid Method for the Identification of Plasmid Deoxyribonucleic Acid in Bacteria, Plasmid, 1978, vol. 1, no.4, pp. 584–588.

    Article  CAS  PubMed  Google Scholar 

  20. Konnova, S.A., Polysaccharide-Containing Polymers in Bacteria of the Genus Azospirillum: Diversity in Chemical Structure and Functions, Extended Abstract of Doctoral (Biology) Dissertation, Bach Inst. Biochem., RAS, 2003.

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049, Russia

    L. P. Petrova, L. Yu. Matora, G. L. Burygin, I. V. Borisov & E. I. Katsy

Authors
  1. L. P. Petrova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Yu. Matora
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. L. Burygin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. V. Borisov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. I. Katsy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. I. Katsy.

Additional information

__________

Translated from Mikrobiologiya, Vol. 74, No. 2, 2005, pp. 224–230.

Original Russian Text Copyright © 2005 by Petrova, Matora, Burygin, Borisov, Katsy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Petrova, L.P., Matora, L.Y., Burygin, G.L. et al. Analysis of DNA, lipopolysaccharide structure, and some cultural and morphological properties in closely related strains of Azospirillum brasilense . Microbiology 74, 188–193 (2005). https://doi.org/10.1007/s11021-005-0050-7

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11021-005-0050-7

Key words

Search

Navigation