Abstract
The paper describes three Penicillium verruculosum 28K mutants with about threefold enhanced production of five industrially important carbohydrases. The two-stage fermentation process that we developed provided a further two- to threefold increase in the production of carbohydrases. Physiological and biochemical studies showed that the synthesis of all five carbohydrases is inducible. Carboxymethylcellulase, xylanase, and β-glucanase are synthesized under a common regulatory control, as is evident from the concurrent increase in the synthesis of these enzymes in the presence of microcrystalline cellulose. The synthesis of avicelase and β-glucosidase is evidently induced by other cellulose- and hemicellulose-containing compounds present in the fermentation medium and, hence, is regulated independently of the three aforementioned enzymes.
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Translated from Mikrobiologiya, Vol. 74, No. 2, 2005, pp. 172–178.
Original Russian Text Copyright © 2005 by Solov’eva, Okunev, Vel’kov, Koshelev, Bubnova, Kondrat’eva, Skomarovskii, Sinitsyn.
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Solov’eva, I.V., Okunev, O.N., Vel’kov, V.V. et al. The selection and properties of Penicillium verruculosum mutants with enhanced production of cellulases and xylanases. Microbiology 74, 141–146 (2005). https://doi.org/10.1007/s11021-005-0043-6
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DOI: https://doi.org/10.1007/s11021-005-0043-6