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MiRNA-34c-5p protects against cerebral ischemia/reperfusion injury: involvement of anti-apoptotic and anti-inflammatory activities

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Abstract

MicroRNAs (miRNAs) are known as important regulators of gene expression and play important roles in diverse biological activities. However, the involvement of miRNAs in cerebral ischemia remains elusive. In the present study, using the middle cerebral artery occlusion (MCAO) model and oxygen-glucose deprivation/reperfusion (OGD/RP)-induced cell injury model, we found that the expression levels of miR-34c-5p were significantly reduced in MCAO rats and OGD/RP cells. Overexpression of miR-34c-5p could improve the increased brain infarction, brain water content and neurological scores in MCAO rats, as well as the abnormal expression of inflammatory cytokines (TNF-α, IL-6, COX-2, iNOS, IL-10) in OGD/RP cells. Moreover, overexpression of miR-34c-5p was found to inhibit the activity of nuclear factor-kappa B (NF-κB) by regulating the expression of nuclear receptor coactivator 1 (NCOA1), and increase the apoptotic rate of cortical neurons by inhibiting the expression of Caspase-3 and Bax and upregulating the expression of Bcl-2. Taken together, our findings demonstrated that miR-34c-5p plays an important role in cerebral ischemia/reperfusion injury, which may be mediated through inflammatory and apoptotic signaling pathways.

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Data availability

The analyzed data sets generated during the study are available from the corresponding author on reasonable request.

Abbreviations

I/R:

Ischemia-Reperfusion

miRNAs:

MicroRNAs

SD:

Sprague-Dawley

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Authors and Affiliations

  1. Trauma Center, Third Affiliated Hospital of Nanchang University, No. 739 Qingshan South Road, Nanchang City, Jiangxi Province, 330000, People’s Republic of China

    Yaoran Tu & Yong Hu

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  1. Yaoran Tu
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  2. Yong Hu
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YRT, YH contributed to data analysis, drafting or revising the article, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.

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Correspondence to Yong Hu.

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All experimental protocols were performed in accordance with the Third Affiliated Hospital of Nanchang University Animal Experimental Guide and approved by the Third Affiliated Hospital of Nanchang University Animal Experimental Ethics Committee.

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The authors declare that they have no competing interests.

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Supplementary Information

Supplementary Figure 1

Full-size immunoblots related to all western blot examples that were shown. (DOCX 166 kb)

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Tu, Y., Hu, Y. MiRNA-34c-5p protects against cerebral ischemia/reperfusion injury: involvement of anti-apoptotic and anti-inflammatory activities. Metab Brain Dis 36, 1341–1351 (2021). https://doi.org/10.1007/s11011-021-00724-5

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  • DOI: https://doi.org/10.1007/s11011-021-00724-5

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