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Telmisartan attenuates hydrogen peroxide-induced apoptosis in differentiated PC12 cells

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Abstract

The present study investigated the protective actions of telmisartan, an angiotensin II type 1 receptor blocker (ARBs), against the cell apoptosis induced by exposure to hydrogen peroxide (H2O2) in differentiated PC12 cells. Preincubation of PC12 cells with telmisartan prevented H2O2-induced cytotoxicity as indicated by increased MTT (3,(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium bromide) reduction, decreased lactate dehydrogenase (LDH) release, and improved morphological changes. Hoechst 33,258 staining showed that telmisartan markedly reduced shrunken nuclei of the cells, and Western blot analysis indicated that telmisartan significantly attenuated caspase-3 activity, as indicated by decreased ratio of cleaved Caspase-3 to its precursor and increased ratio of Bcl-2/Bax. The present findings showed that telmisartan protected against cellular oxidative damages by inhibiting apoptotic response.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (81573413 and 81773714 to Hao Hong) and the Fundamental Research Funds for the Central Universities (2632017PT01).

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  1. Guan Tao Du and Xuan Ke contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmacy, Department of Endocrinology, Changzhou No. 2 People’s Hospital Affiliated with Nanjing Medical University, Changzhou, 213003, China

    Guan Tao Du, Guang Jun Liu, Hui Ying Wu, Jin Hong Gong, Xiao Dan Qian & Jin Luo Cheng

  2. Department of Pharmacology, China Pharmaceutical University, Nanjing, 210009, China

    Xuan Ke & Hao Hong

  3. School of Pharmacy, Nantong University, Nantong, 226001, Jiangsu, China

    Guo Liang Meng

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  1. Guan Tao Du
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  2. Xuan Ke
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  4. Guang Jun Liu
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Correspondence to Jin Luo Cheng or Hao Hong.

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Du, G.T., Ke, X., Meng, G.L. et al. Telmisartan attenuates hydrogen peroxide-induced apoptosis in differentiated PC12 cells. Metab Brain Dis 33, 1327–1334 (2018). https://doi.org/10.1007/s11011-018-0237-z

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