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Vanillic acid attenuates cerebral hyperemia, blood-brain barrier disruption and anxiety-like behaviors in rats following transient bilateral common carotid occlusion and reperfusion

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Abstract

Transient bilateral common carotid artery occlusion (tBCCAO), followed by reperfusion, is a model of transient global hypoperfusion. In the present study we aimed to investigate the probable effects of Vanillic acid (VA) on some physiological parameters including cerebral hyperemia, blood-brain barrier (BBB) disruption, anxiety behaviors and neurological deficits induced by bilateral occlusion of the common carotid arteries and reperfusion (BCCAO/R) in rats. Rats were randomly divided into four groups; Sham, BCCAO/R, VA and VA+ BCCAO/R. Chronic cerebral hypoperfusion was induced after 2 weeks of pretreatment by VA. Subsequently, sensorimotor scores, elevated plus maze tests, cerebral hyperemia, and BBB disruption were evaluated 72 h after 30 min of BCCAO. Pretreatment of rats by VA improved sensory motor signs, anxiolytic behavior in BCCAO/R rats compared with untreated rats (p < 0.05). Further, VA attenuated reactive hyperemia and BBB disruption in BCCAO/R rats compared with untreated rats (p < 0.01). To our knowledge, this study is the first to reveal VA could attenuate reactive hyperemia and improve BBB disruption following BCCAO/R, and could improve neurological scores and anxiety like behaviors in this model of cerebral hypoperfusion. These results suggest that VA could be a promising pretreatment agent in cerebral hypoperfusion.

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Acknowledgements

This paper was extracted as a part of Seyed Esmaeil Khoshnam’s Ph.D. thesis. The study was financially supported by research affairs of Ahvaz Jundishapur University of Medical Sciences (Grant No. APRC-95-17). Furthermore, the authors would especially like to thank Professor William Winlow for outstanding editing and proof reading.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Seyed Esmaeil Khoshnam, Yaghoob Farbood, Hadi Fathi Moghaddam, Alireza Sarkaki & Mohammad Badavi

  2. Cell & Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Layasadat Khorsandi

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  1. Seyed Esmaeil Khoshnam
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  2. Yaghoob Farbood
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Khoshnam, S.E., Farbood, Y., Fathi Moghaddam, H. et al. Vanillic acid attenuates cerebral hyperemia, blood-brain barrier disruption and anxiety-like behaviors in rats following transient bilateral common carotid occlusion and reperfusion. Metab Brain Dis 33, 785–793 (2018). https://doi.org/10.1007/s11011-018-0187-5

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