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Contributions of glycogen to astrocytic energetics during brain activation

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Abstract

Glycogen is the major store of glucose in brain and is mainly in astrocytes. Brain glycogen levels in unstimulated, carefully-handled rats are 10–12 μmol/g, and assuming that astrocytes account for half the brain mass, astrocytic glycogen content is twice as high. Glycogen turnover is slow under basal conditions, but it is mobilized during activation. There is no net increase in incorporation of label from glucose during activation, whereas label release from pre-labeled glycogen exceeds net glycogen consumption, which increases during stronger stimuli. Because glycogen level is restored by non-oxidative metabolism, astrocytes can influence the global ratio of oxygen to glucose utilization. Compensatory increases in utilization of blood glucose during inhibition of glycogen phosphorylase are large and approximate glycogenolysis rates during sensory stimulation. In contrast, glycogenolysis rates during hypoglycemia are low due to continued glucose delivery and oxidation of endogenous substrates; rates that preserve neuronal function in the absence of glucose are also low, probably due to metabolite oxidation. Modeling studies predict that glycogenolysis maintains a high level of glucose-6-phosphate in astrocytes to maintain feedback inhibition of hexokinase, thereby diverting glucose for use by neurons. The fate of glycogen carbon in vivo is not known, but lactate efflux from brain best accounts for the major metabolic characteristics during activation of living brain. Substantial shuttling coupled with oxidation of glycogen-derived lactate is inconsistent with available evidence. Glycogen has important roles in astrocytic energetics, including glucose sparing, control of extracellular K+ level, oxidative stress management, and memory consolidation; it is a multi-functional compound.

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Abbreviations

CMRglc :

Rate of glucose utilization

CMRO2 :

Rate of oxygen utilization

DG:

2-deoxy-D-glucose

DG-1-P:

Deoxyglucose-1-phosphate

DG-6-P:

Deoxyglucose-6-phosphate

Glc:

Glucose

Glc-6-P:

Glucose-6-phosphate

Lac:

Lactate

Pyr:

Pyruvate

TCA:

Tricarboxylic acid

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Acknowledgments

This work was supported, in part, by National Institutes of Health grants DK081936, NS038230, and NS36728. The authors declare no conflict of interest.

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  1. Department of Neurology, University of Arkansas for Medical Sciences, Slot 500, 4301 W. Markham St., Little Rock, AR, 72205, USA

    Gerald A. Dienel & Nancy F. Cruz

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Dienel, G.A., Cruz, N.F. Contributions of glycogen to astrocytic energetics during brain activation. Metab Brain Dis 30, 281–298 (2015). https://doi.org/10.1007/s11011-014-9493-8

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