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Iron-dextran injection into the substantia nigra in rats decreases striatal dopamine content ipsilateral to the injury site and impairs motor function

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Abstract

Iron chloride injections into the rat SNc can cause chronic decreases in striatal dopamine (DA) levels. However, changes in striatal DA content after iron-dextran injection into rat SNc have not been completely elucidated. The aim of this work was to measure striatal DA concentrations after iron-dextran injection into the SNc. We divided 40 male Wistar rats into five groups, including control, saline injected then sacrificed 7 days or 30 days later, and iron-dextran injected then sacrificed 7 days or 30 days later. Striatal DA content was measured in control animals and in all animals sacrificed 7 days or 30 days after injection, and motor performance was assessed in iron-dextran and saline injected groups 30 days after injection. The striatal DA levels were determined using HPLC. There were significant (P < 0.05) decreases in DA concentrations in the striatum ipsilateral to the injection site in the iron-dextran treated rats compared to control and saline-injected rats. There were no significant differences in DA concentration between the sham-operated (i.e., saline-injected) and control rats. We also observed motor deficits in the iron-dextran injected rats. The striatal DA reduction observed after iron-dextran injection may be attributable to iron-induced oxidative injury in the SNc. Motor deficits, in turn, may be explained by subsequent disturbances in striatal and cortical dopaminergic neuromodulation.

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Authors and Affiliations

  1. Departamento de Neurofisiologia-Laboratorio de Cromatografia y Microdialisis, Instituto Nacional de Rehabilitacion, SSA, Mexico City D.F., Mexico

    Antonio Bueno-Nava

  2. Departamento de Neurofisiologia-Laboratorio de Neuroplasticidad, Instituto Nacional de Rehabilitacion, SSA, Mexico City D.F., Mexico

    Rigoberto Gonzalez-Pina

  3. Departamento de Neurofisiologia-Laboratorio de Neuroquimica, Torre de Investigacion, Instituto Nacional de Rehabilitacion, SSA, Calz. México-Xochimilco 289, Col. Arenal de Guadalupe Deleg. Tlalpan, C.P. 14389, Mexico City, Mexico

    Alfonso Alfaro-Rodriguez

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  1. Antonio Bueno-Nava
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  2. Rigoberto Gonzalez-Pina
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  3. Alfonso Alfaro-Rodriguez
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Correspondence to Alfonso Alfaro-Rodriguez.

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Bueno-Nava, A., Gonzalez-Pina, R. & Alfaro-Rodriguez, A. Iron-dextran injection into the substantia nigra in rats decreases striatal dopamine content ipsilateral to the injury site and impairs motor function. Metab Brain Dis 25, 235–239 (2010). https://doi.org/10.1007/s11011-010-9200-3

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  • DOI: https://doi.org/10.1007/s11011-010-9200-3

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