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The role of ubiquitin signaling pathway on liver regeneration in rats

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Abstract

The ubiquitin signalling pathway is a large system associated with numerous intracellular mechanisms. However, its function in the liver regeneration process remains unknown. This particular study investigates the intracellular effect mechanisms of the ubiquitin signalling pathway. It also determines the differences in the expression of 88 genes belonging to the ubiquitin pathway using the RT-PCR array method. To conduct this research, three genes—that differed in the expression analysis were selected. Moreover, their proteins were analysed by western blot analysis while using Ki67 immunohistochemical analysis that determines proliferation rates by hour. It was determined that BRCA1 and BARD1, which are effective in DNA repair, play an active role at PH24. Similarly, Ube2t expression, which belongs to the Fanconi anaemia pathway associated with DNA repair, was also found to be high at PH12-72 h. In addition, it was revealed that the expressions of Anapc2, Anapc11, Cdc20 belonging to the APC/CCdc20 complex, which participate in cell cycle regulation, differed at different hours after PH. Expression of Mul1, which is involved in mitochondrial biogenesis and mitophagy mechanisms, peaked at PH12 under the observation. Considering the Mul1 gene expression difference, MUL1-mediated mitophagy and mitochondrial fission mechanism may be associated with liver regeneration. It was also determined that PARKIN-mediated mitophagy mechanisms are not active in 0–72 h of liver regeneration since PARKIN expression did not show a significant change in PH groups.

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Data availability

The online version of RT-PCR Array data (unnormalized/normalised/fold change) available at: https://github.com/aaozmen/aaozmen

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Acknowledgements

Thanks to Prof. Dr. Didem TURGUT COŞAN for her valuable contributions

Funding

This study is funded by Eskisehir Osmangazi University Project Office, Project number 201719040.

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Science, Hitit University, 19030, Corum, Turkey

    Ayse Ozmen Yaylaci

  2. Department of Biology, Faculty of Arts and Science, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey

    Mediha Canbek

Authors
  1. Ayse Ozmen Yaylaci
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  2. Mediha Canbek
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Contributions

AOY Writing the article, Gene expression analysing, experimental design, Western blotting, rats surgery. MC Funding acquisition, project administration, Supervision, doing histological section and participated in discussion.

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Correspondence to Ayse Ozmen Yaylaci.

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Ozmen Yaylaci, A., Canbek, M. The role of ubiquitin signaling pathway on liver regeneration in rats. Mol Cell Biochem 478, 131–147 (2023). https://doi.org/10.1007/s11010-022-04482-5

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  • DOI: https://doi.org/10.1007/s11010-022-04482-5

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