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RETRACTED ARTICLE: MiRNA-27a mediates insulin resistance in 3T3-L1 cells through the PPARγ

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This article was retracted on 28 June 2023

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Abstract

The biological actions of insulin have been originated by activation of membrane receptors, which trigger a diversity of signaling pathways in facilitating their biological activities. Insulin homeostasis functions in promoting metabolism balance and promotes cell growth and proliferation. If these mechanisms are reformed, this could lead to insulin resistance as a result of defective insulin signaling triggered by mutations in receptors or effector molecules located downstream or by abnormal posttranslational modifications. The purpose of this is to preliminarily investigate the mechanism of miRNA-27a-mediating insulin resistance in 3T3-L1 cells. Insulin resistance in 3T3-L1 adipocytes as a cell model was induced by tumor necrosis factor-alpha (TNF-α) and the miRNA-27a expression in 3T3-L1 adipocytes had been experiential. The regulation of peroxisome proliferator-activated receptor-gamma (PPARγ) mRNA by miRNA-27a had been studied by reverse transcription receptor polymerase chain reaction (RT-PCR). MiRNA-27a was up-regulated in 3T3-L1 cells, miRNA-27a mimics reserved expression of PPARγ mRNA, and miRNA-27a inhibitors up-regulated the expression of PPARγ mRNA. The insulin resistance in 3T3-L1 cells mediated by miRNA-27a may be achieved by targeting PPARγ.

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The data that support the findings of this study are available on request from the corresponding author.

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This research received no funding.

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Authors and Affiliations

  1. Department of Endocrinology, Beijing Tongrentang Hospital of Traditional Chinese Medicine, 23 Hou Str., Beijing, 100051, People’s Republic of China

    Yangming Zhuang

  2. Department of Endocrinology, The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, 24 Heping Rd, Harbin, 150040, Heilongjiang, People’s Republic of China

    Ming Li

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  1. Yangming Zhuang
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Contributions

YZ assisted in writing, interpretation, reviewing of the article, and editing of the manuscript. ML assisted in concept, design, data collection, data analysis, and writing of the original draft.

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Correspondence to Yangming Zhuang.

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The study was carried out at the Beijing Tongrentang Hospital of Traditional Chinese Medicine (Beijing, People’s Republic of China) and was approved by the Local Ethics Committee.

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This article has been retracted. Please see the retraction notice for more detail:https://doi.org/10.1007/s11010-023-04799-9

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Zhuang, Y., Li, M. RETRACTED ARTICLE: MiRNA-27a mediates insulin resistance in 3T3-L1 cells through the PPARγ. Mol Cell Biochem 477, 1107–1112 (2022). https://doi.org/10.1007/s11010-022-04367-7

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  • DOI: https://doi.org/10.1007/s11010-022-04367-7

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