Abstract
Bone marrow mesenchymal stem cells (BMSCs) are multipotential differentiation cells which can differentiate into different cell types such as osteoblasts, chondrocytes, adipocytes, cardiomyocytes, hepatocytes, endothelial cells, and neuronal cells. Such multipotential differentiation makes them attractive for stem cell-based therapy aimed at treating previously incurable disorders. In the present work, we encapsulated BMSCs into a hydrogel with a three-dimensional (3D) network of nanofibers, formed from self-assembling of peptide amphiphile. The self-assembling of peptide amphiphile into hydrogel was triggered by mixing cell suspensions with dilute aqueous solutions of amphipathic peptide. Moreover, this hydrogel was designed to present cells the neurite-promoting laminin epitope IKVAV at nearly van der Waals density, which induced the successful differentiation of BMSCs into neural cells.
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Acknowledgements
The authors thank the faculty and the students of the Master in Department of Orthopedics Surgery, The Second Affiliated Hospital of Nanchang University, for feedback and comments on this work.
Funding
Supported by the National Natural Science Foundation of China(Spinal cord tissue engineering module fabricated by three-dimensional printing to promoting regeneration of injured spinal cord; Tissue-Engineered Vascular Constructs by Branched Peptide to Promote the Regeneration after Spinal Cord Injury) (Nos. 30801158, 81360271); the Natural Science Foundation of Jiangxi Province (Construction of vascularized bone tissue engineering by three-dimensional printing to improve regeneration of bone defect; Polypeptide matrix combined with transgenic neural stem cells to improve the regeneration of spinal cord injury; Biomimetic branched peptide matrix modified by gene to improve the regeneration of bone defect) (Nos. 20151BAB205051, 20151BAB205055, 20142BAB205066); the Education Department of Jiangxi Province (Vascular branched peptide-based tissue engineering construction modified with NT-3 and VEGF gene; Experimental study of neural stem cells induced by peptide self-assembly scaffold in vitro) (Nos. GJJ09086, GJJ14054).
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Ruan, H., Xiao, R., Jiang, X. et al. Biofunctionalized self-assembly of peptide amphiphile induces the differentiation of bone marrow mesenchymal stem cells into neural cells. Mol Cell Biochem 450, 199–207 (2019). https://doi.org/10.1007/s11010-018-3386-9
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DOI: https://doi.org/10.1007/s11010-018-3386-9