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Molecular and biochemical evidence on the protective role of ellagic acid and silybin against oxidative stress-induced cellular aging

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Abstract

Aging is a natural process in living organisms that is defined by some molecular and cellular changes with time. Various causes such as mitochondrial DNA aberrations, aggregation of proteins, telomere shortening, and oxidative stress have an influential role in aging of the cells. Natural antioxidants are compounds that are potent to protect the body from detrimental effects of molecules such as free radicals. The aim of this study was to evaluate the anti-aging properties of ellagic acid (EA) and silybin (SIL), as natural antioxidant compounds on rat embryonic fibroblast (REF) cells. These cells were pre-incubated with EA and SIL, thereafter were exposed to hydrogen peroxide (H2O2). Then, the cell viability, SA-β-GAL activity, distribution of cell cycle, NF-κB, and mitochondrial complex I, II/IV enzyme activity were measured. The results of this study revealed the protective effects of EA and SIL in H2O2-treated REF cells, which confirm the previous achieved data on antioxidant and anti-inflammatory characteristics of EA and SIL against H2O2 in the treated REF cells. However, more new in vivo experiments are required to discover the anti-aging effects and mechanism of action of such compounds.

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Acknowledgements

This study was in part supported by the personal grant of the corresponding author from TUMS (ID: 94-01-45-28956) and the assistance of the INSF.

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  1. Maryam Baeeri and Solmaz Mohammadi-Nejad have contributed equally to this work.

Authors and Affiliations

  1. Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Baeeri, Solmaz Mohammadi-Nejad, Mahban Rahimifard, Mona Navaei-Nigjeh, Shermineh Moeini-Nodeh, Reza Khorasani & Mohammad Abdollahi

  2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mona Navaei-Nigjeh

  3. International Campus, Tehran University of Medical Sciences (TUMS-IC), Tehran, Iran

    Mohammad Abdollahi

  4. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Abdollahi

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  1. Maryam Baeeri
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Baeeri, M., Mohammadi-Nejad, S., Rahimifard, M. et al. Molecular and biochemical evidence on the protective role of ellagic acid and silybin against oxidative stress-induced cellular aging. Mol Cell Biochem 441, 21–33 (2018). https://doi.org/10.1007/s11010-017-3172-0

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