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Association of single nucleotide polymorphisms of IL23R and IL17 with necrotizing enterocolitis in premature infants

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Abstract

Necrotizing enterocolitis (NEC) is a severe gastrointestinal inflammatory disease in neonates, particularly in preterm infants. The interleukin (IL) 23/IL17 axis has been shown to play an important role in the gastrointestinal inflammation. However, the association of gene polymorphisms in the IL23/IL17 axis and the development of NEC remains unknown. In this study, we aimed to explore a possible genetic role of IL23R and IL17 in the development of NEC. We identified single nucleotide polymorphisms (SNPs) in IL23R (rs10889677), IL17A (rs2275913), and IL17F (rs763780) by polymerase chain reaction and Sanger sequencing. A total of 102 NEC patients (stage II, n = 75; and stage III, n = 27) and 120 control subjects were recruited for the study. All of the participants were premature (gestational age < 37 weeks). Our results revealed that the combination of the IL17F rs763780 (TC + CC) genotype and the C allele both significantly increased the risk of NEC [odds ratio (OR) 1.89, 95% confidence interval (CI) 1.04–3.43, P = 0.035; OR 1.82, 95% CI 1.06–3.13, P = 0.028, respectively]. Furthermore, the rs763780 (TC + CC) genotype was associated with increased severity of NEC and the incidence of NEC-related perforation [OR 2.80, 95% CI 1.10–7.12, P = 0.031; OR 3.86, 95% CI 1.10–13.53, P = 0.035, respectively]. However, IL23R rs10889677 and IL17A rs2275913 were not associated with the susceptibility to NEC. In conclusion, our data suggest that a variant of IL17F (rs763780) may contribute to the development of NEC.

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Acknowledgements

Yanjun Liu is supported by NIH grant SC1 DK104821.

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Authors and Affiliations

  1. Department of Neonatology, The First Hospital of Jilin University, Changchun, China

    Jiayi Tian, Yanjun Liu, Tong Zhu & Chaoying Yan

  2. Division of Endocrinology, Metabolism, and Molecular Medicine, Charles R. Drew University of Medicine and Sciences, University of California Los Angeles (UCLA) School of Medicine, Los Angeles, CA, USA

    Yanjun Liu

  3. Department of Central Laboratory, The First Hospital of Jilin University, Changchun, China

    Jiayi Tian, Yanfang Jiang, Haohan Zhou & Liping Peng

  4. Department of Orthopedics Surgery, The Second Hospital of Jilin University, Changchun, China

    Haohan Zhou

  5. Department of Respirology, The First Hospital of Jilin University, Changchun, China

    Liping Peng

  6. Department of Neonatology, The Second Hospital of Jilin University, Changchun, China

    Xiaoqi Zhao

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  1. Jiayi Tian
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Correspondence to Liping Peng or Chaoying Yan.

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Tian, J., Liu, Y., Jiang, Y. et al. Association of single nucleotide polymorphisms of IL23R and IL17 with necrotizing enterocolitis in premature infants. Mol Cell Biochem 430, 201–209 (2017). https://doi.org/10.1007/s11010-017-2972-6

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  • DOI: https://doi.org/10.1007/s11010-017-2972-6

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