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Association of endothelial nitric oxide synthase (eNOS) gene G894T polymorphism with hypertension risk and complications

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Abstract

This study evaluated the association of NOS3 polymorphisms with hypertension risk and complications. eNOS (G894T) SNP was performed by RT-PCR on 70 hypertensive patients (25 were hypertensive, 25 were hypertensive with CAD, and 20 were diabetic with hypertension) and 30 age- and gender-matched individuals. Lipid and glucose profile were assessed by standard colorimetric assay. Our results revealed that combination of (GT + TT) genotype and T allele significantly increases the risk of hypertension (OR = 3.86 and 4.33), respectively. Subgroup analysis showed significant association between CAD with eNOS (G894T) mutant genotype (P = 0.002) and allele frequency (P < 0.001). Moreover, the mutant homozygous and heterozygous eNOS genotype together were significantly associated with higher TC, LDLc, (P < 0.001), and TG (P = 0.001). Thus, hypercholesterolemia (P < 0.001 and OR = 12.48) increases the risk of hypertension among T carrier. These results indicated that the T carriers significantly increase hypertension risk and complication (CAD), mainly with hypercholesterolemia and in elderly.

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Authors’ contribution

Alrefai A and Habib M researched literature and conceived the study. Yaseen R, Habib M and Habeeb R gained ethical approval, and contributed to data collection and analysis. Alrefai A wrote the first draft of the manuscript. ALrefai A and Habib M reviewed and edited the manuscript and all authors approved the final version of this manuscript.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt

    Abeer A. ALrefai & Mona Salah El-din Habib

  2. Departments of Cardiology, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt

    Rehab I. Yaseen & Mahmoud K. Gabr

  3. Departments of Anesthesia, Faculty of Medicine, Menoufia University, Shebeen El-Kom, Egypt

    Rabab M. Habeeb

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  1. Abeer A. ALrefai
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  2. Mona Salah El-din Habib
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Correspondence to Abeer A. ALrefai.

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This study has been approved by ethical committee for research of Faculty of Medicine- Menoufia University- Egypt.

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ALrefai, A.A., Habib, M.S.Ed., Yaseen, R.I. et al. Association of endothelial nitric oxide synthase (eNOS) gene G894T polymorphism with hypertension risk and complications. Mol Cell Biochem 421, 103–110 (2016). https://doi.org/10.1007/s11010-016-2790-2

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  • DOI: https://doi.org/10.1007/s11010-016-2790-2

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