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Redox status in acute ischemic stroke: correlation with clinical outcome

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Abstract

Connection between oxidative stress and clinical outcome in acute ischemic stroke (AIS) has been poorly investigated. This study was aimed to assess redox state (through measurement of oxidative stress markers) of patients with acute ischemic stroke during different stages of follow-up period, and to find association between values of mentioned markers and clinical outcome. The investigation was conducted on 60 patients (both sexes, aged 75.90 ± 7.37 years) who were recruited in intensive care units at the Special Hospital for Cerebrovascular Diseases “Sveti Sava,” Belgrade. After verification of AIS, patients were followed up in four interval of time: (1) at admission, (2) within 24 h after AIS, (3) within 72 h after AIS, and (4) 7 days after AIS. At these points of time, blood samples were taken for determination of oxidative stress parameters [index of lipid peroxidation (measured as TBARS), nitric oxide (NO) in the form of nitrite (\({\text{NO}}_2^-\)), superoxide anion radical (\({\text{O}}_2^ -\)), hydrogen peroxide (H2O2)], and enzymes of antioxidant defense system [superoxide dismutase (SOD) and catalase (CAT)] using spectrophotometer. Present study provides new insights into redox homeostasis during ischemic stroke which may be of interest in elucidation of molecular mechanisms involved in this life-threatening condition. Particular contribution of obtained results could be examination of connection between redox disruption and clinical outcome in these patients. In that sense, our finding have pointed out that \({\text{O}}_2^ -\) and NO can serve as the most relevant adjuvant biomarkers to monitor disease progression and evaluate therapies.

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Acknowledgments

This work is supported by Grant no. 175043 from the Ministry of Science and Technical Development of the Republic of Serbia.

Conflict of interest

All authors of the present paper disclose no actual or potential conflicts of interest, including any financial, personal, or other relationships with people or organizations.

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Authors and Affiliations

  1. Institute of Gerontology and Palliative Care, Belgrade, Republic of Serbia

    Dalibor Paspalj

  2. Special Hospital for Prevention and Treatment of Cerebrovascular Diseases “Sveti Sava”, Belgrade, Republic of Serbia

    Petar Nikic & Milan Savic

  3. Institute of Medical Physiology “Richard Burian”, School of Medicine, University of Belgrade, Belgrade, Republic of Serbia

    Dragan Djuric

  4. Specialized Hospital for Rehabilitation and Orthopedic Prosthetics, Belgrade, Serbia

    Igor Simanic

  5. Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 69 Svetozara Markovica, 34000, Kragujevac, Republic of Serbia

    Vladimir Zivkovic, Ivan Srejovic & Vladimir Jakovljevic

  6. Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Republic of Serbia

    Nevena Jeremic

Authors
  1. Dalibor Paspalj
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  2. Petar Nikic
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  3. Milan Savic
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  4. Dragan Djuric
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  6. Vladimir Zivkovic
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  7. Nevena Jeremic
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  8. Ivan Srejovic
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  9. Vladimir Jakovljevic
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Correspondence to Vladimir Jakovljevic.

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Paspalj, D., Nikic, P., Savic, M. et al. Redox status in acute ischemic stroke: correlation with clinical outcome. Mol Cell Biochem 406, 75–81 (2015). https://doi.org/10.1007/s11010-015-2425-z

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  • DOI: https://doi.org/10.1007/s11010-015-2425-z

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