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Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury

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Abstract

Hyperhomocysteinemia is a risk factor for cardiovascular disease and the mechanism of homocysteine (HCY)-induced vascular endothelial cell injury has been intensively studied for many years. Recently, a large number of studies have shown inhibitory effects of genistein (GEN), a soy isoflavone, in the process of endothelial cell injury. In the present study, the protective effects of GEN in HCY-induced endothelial cell inflammatory injury were investigated. A model of HCY-induced endothelial cell (ECV-304) inflammatory injury was established in vitro, and the protective effect of GEN in this procession was explored. According to our results, GEN protected HCY-induced endothelial cell from viability decreases, meanwhile prevented the changes of cell morphology and the production of reactive oxygen species (ROS). The expression of NF-kB P-65, IL-6, and ICAM-1 was all down-regulated. During the HCY-induced endothelial cell injury, the endothelial cell apoptosis and proliferation disorder were alleviated. Therefore, we conclude that HCY-induced endothelial cell inflammatory injury could be blocked by GEN. The present findings suggest that GEN protects HCY-induced endothelial cell inflammatory injury may through reducing the release of ROS, inhibiting NF-kB activation, down-regulating the expression of cytokine IL-6 and adhesion molecules ICAM-1, avoiding inflammatory cells and platelet adhesion, accordingly, leading to a balance of endothelial cell proliferation and apoptosis.

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Authors and Affiliations

  1. Clinical Laboratory of Zhengzhou Traditional Chinese Medicine Hospital, Wenhuagong Road 65, Zhengzhou, 450007, China

    Shengbo Han & Pan Gao

  2. School of Public Health, Xinxiang Medical University, East of JinSui Road of XinXiang City, Xinxiang, 453003, Henan, China

    Hui Wu

  3. Clinical Laboratory of The Eighth People’s Hospital of Zhengzhou City, Ruimin street, Zhengzhou, 450006, China

    Wenxue Li

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  1. Shengbo Han
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  2. Hui Wu
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  4. Pan Gao
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Correspondence to Shengbo Han.

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Han, S., Wu, H., Li, W. et al. Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury. Mol Cell Biochem 403, 43–49 (2015). https://doi.org/10.1007/s11010-015-2335-0

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