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Gene expression profiling of endometrium versus bone marrow-derived mesenchymal stem cells: upregulation of cytokine genes

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Abstract

Postulated Stem/progenitor cells involved in endometrium regeneration are epithelial, mesenchymal, and endothelial. Bone marrow (BM) has been implicated in endometrial stem cells. We aimed at studying gene expression profiling of endometrial mesenchymal stem cells compared to BM MSCS to better understand their nature and functional phenotype. Endometrial tissues were obtained from premenopausal hysterectomies (n = 3), minced and enzymatically digested as well as Normal BM aspirates (n=3). Immunophenotyping, differentiation to mesoderm, and proliferation were studied. The expression profile of 84 genes relevant to mesenchymal stem cells was performed. Fold change calculations were determined with SA Biosciences data analysis software. VEGF, G-CSF, and GM-CSF in cultures supernatants of MSCs were assayed by Luminex immunoassay. Endo MSCs possess properties similar to BM MSCs. Cumulative population doubling was significantly higher in Endo MSCs compared to BM MSCs (p < 0.001). 52 core genes were shared between both generated MSCs including stemness, self-renewal, members of the Notch, TGFB, FGF, and WNT.16 downregulated genes (VCAM, IGF1)and 16 upregulated in Endo MSCs compared to BM (p < 0.05 → fourfolds). They included mostly cytokine and growth factor genes G-CSF, GM-CSF, VWF, IL1b, GDF15, and KDR. VEGF and G-CSF levels were higher in Endo MSCs supernatants (p < 0.0001). Cells sharing MSC and endothelial cell characteristics could be isolated from the human endometrium. Endo MSCs share a core genetic profile with BM MSCs including stemness. They show upregulation of genes involved in vasculogenesis, angiogenesis, cell adhesion, growth proliferation, migration, and differentiation of endothelial cells, all contributing to endometrial function.

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Acknowledgments

This work has been sponsored by RAMSES an FP7-REGPOT—project (No. 245691). Authors would like to thank Dr Alshayma Ibrahim [PhD] for cell culture assessment and scientist Omar Adel for molecular biology techniques assessment.

Conflict of interest

Authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University [Stem Cell Research Unit, Pediatric Hospital, Cairo University], Kasr El Aini, Cairo, 11562, Egypt

    Taghrid Gaafar & Rabab El Hawary

  2. Obstetrics and Gynecology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    Omneya Osman & Amira Osman

  3. Pediatric Cardiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    Wael Attia & Hala Hamza

Authors
  1. Taghrid Gaafar
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  2. Omneya Osman
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  3. Amira Osman
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  4. Wael Attia
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  5. Hala Hamza
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  6. Rabab El Hawary
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Correspondence to Taghrid Gaafar.

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Gaafar, T., Osman, O., Osman, A. et al. Gene expression profiling of endometrium versus bone marrow-derived mesenchymal stem cells: upregulation of cytokine genes. Mol Cell Biochem 395, 29–43 (2014). https://doi.org/10.1007/s11010-014-2109-0

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  • DOI: https://doi.org/10.1007/s11010-014-2109-0

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