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Studies on microRNAs that are correlated with the cancer stem cells in chronic myeloid leukemia

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Abstract

Accumulating data indicate that cancer stem cells play an important role in tumorigenesis and are underlying cause of tumor recurrence and metastasis, specifically in chronic myeloid leukemia (CML). We aim to detect the miRNAs that are correlated with the cancer stem cells in CML to provide theoretical basis for clinical application. We first analyzed microRNA expression profiles of CML leukemia patients compared with normal controls by microarray analysis and validated the results by real-time PCR. A single microRNA signature classified CML from normal was detected. We also determined the absolute copy numbers of these three microRNAs in normal adults. The results showed that three microRNAs (miR-150, miR-23a, and miR-130a) were identified to significantly decrease in expanded 38 CML patients compared with 90 normal controls. Molecular and statistical analysis showed that the decreased microRNAs were significant in clinical analysis. All these results indicated that those three microRNAs could act as a tumor suppressor and their decreased expression might be one of the causes of leukemia. Accordingly, clarifying their regulatory mechanisms might delineate their potentials as drug targets of gene therapy for CML.

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Abbreviations

CML:

Chronic myeloid leukemia

Ph:

Philadelphia

AP:

Ammonium persulfate

PHA:

Polyhydroxyalkanoates

MNC:

Mononuclear cell

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Acknowledgments

The research is funded by the National Science Foundation (81100366) and Beijing Nova Programme (2013041).

Conflict of interest

There is no conflict of interest to declare.

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Authors and Affiliations

  1. Clinical Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524001, China

    Xishan Zhu, Ziying Lin, Xu Zhou, Lawei Yang & Gang Liu

  2. Department of Urology, Weifang Traditional Chinese Medicine Hospital, Weifang, China

    Jing Du

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  1. Xishan Zhu
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  2. Ziying Lin
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  3. Jing Du
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  4. Xu Zhou
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  5. Lawei Yang
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  6. Gang Liu
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Correspondence to Gang Liu.

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Zhu, X., Lin, Z., Du, J. et al. Studies on microRNAs that are correlated with the cancer stem cells in chronic myeloid leukemia. Mol Cell Biochem 390, 75–84 (2014). https://doi.org/10.1007/s11010-013-1958-2

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  • DOI: https://doi.org/10.1007/s11010-013-1958-2

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