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RACK1 promotes the proliferation of THP1 acute myeloid leukemia cells

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Abstract

The receptor for activated C kinase 1 (RACK1), an adaptor protein implicated in the regulation of multiple signaling pathways, has been reported to contribute to the survival of leukemic progenitor cells by enhancing the activity of glycogen synthase kinase 3β (GSK3β). However, it remains unknown whether RACK1 also contributes to the oncogenic growth of acute myeloid leukemia (AML) cells. Here, we report that transient or stable silencing of endogenous RACK1 expression by RACK1 short hairpin RNAs (shRNAs) led to impaired proliferation of THP1 AML cells without inducing terminal differentiation. Further exploration revealed that RACK1 loss-of-function resulted in reduced GSK3β activity. GSK3β shRNA treatment showed similar effects to RACK1 loss-of-function. Our data collectively suggest that RACK1 contributes to THP1 cell proliferation through, at least partially, enhancing GSK3β activity. Thus, targeting RACK1 may have some important therapeutic implications in the treatment of AML.

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (31270960, 31100544), the National Key Technologies R&D Program for New Drugs (2013ZX09103003-010), and National Key Basic Research Program of China (2010CB911904).

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Authors and Affiliations

  1. Department of Immunology, College of Basic Medical Sciences, Central South University, Changsha, 410078, People’s Republic of China

    Dalin Zhang, Ting Zhu & Jiyan Zhang

  2. Department of Molecular Immunology, Institute of Basic Medical Sciences, 27 Taiping Road, Beijing, 100850, People’s Republic of China

    Dalin Zhang, Qingyang Wang, Ting Zhu, Junxia Cao, Xueying Zhang, Jing Wang, Xiaoqian Wang, Yan Li, Beifen Shen & Jiyan Zhang

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  1. Dalin Zhang
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  2. Qingyang Wang
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  3. Ting Zhu
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  4. Junxia Cao
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  5. Xueying Zhang
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  6. Jing Wang
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  7. Xiaoqian Wang
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  8. Yan Li
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  9. Beifen Shen
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  10. Jiyan Zhang
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Corresponding author

Correspondence to Jiyan Zhang.

Additional information

Dalin Zhang and Qingyang Wang have contributed equally to this study.

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Zhang, D., Wang, Q., Zhu, T. et al. RACK1 promotes the proliferation of THP1 acute myeloid leukemia cells. Mol Cell Biochem 384, 197–202 (2013). https://doi.org/10.1007/s11010-013-1798-0

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