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MicroRNA-200b targets CREB1 and suppresses cell growth in human malignant glioma

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Abstract

MicroRNAs can coordinately repress multiple target genes and interfere with the biological functions of the cell, such as proliferation and apoptosis. In the present study, we report that miR-200b was downregulated in malignant glioma cell lines and specimens. Overexpression of miR-200b suppressed the proliferation and colony formation of glioma cells. An oncogene encoding cAMP responsive element-binding protein 1 (CREB1), which has been shown to be an important transcription factor involved in the proliferation, survival, and metastasis of tumor cells, was here confirmed as a direct target gene of miR-200b. CREB1 was also found to be present at a high level in human glioma tissues. This was inversely correlated with miR-200b expression. Ectopic expression of CREB1 attenuated the growth suppressive phenotypes of glioma cells caused by miR-200b. These results indicate that miR-200b targets the CREB1 gene and suppresses glioma cell growth, suggesting that miR-200b shows tumor-suppressive activity in human malignant glioma.

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Acknowledgments

This study was supported by the Basilic Special Financial Support of Affiliated Cancer Hospital Guangzhou Medical University (2010-yz-01).

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Authors and Affiliations

  1. Affiliated Cancer Hospital, Guangzhou Medical University, Guangzhou, Guangdong, People’s Republic of China

    Biao Peng, Su Hu, Qinming Jun, Dongdong Luo, Xun Zhang, Hailin Zhao & Dan Li

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  1. Biao Peng
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  2. Su Hu
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  3. Qinming Jun
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  4. Dongdong Luo
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  5. Xun Zhang
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  6. Hailin Zhao
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  7. Dan Li
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Corresponding author

Correspondence to Biao Peng.

Additional information

Biao Peng and Su Hu contributed equally to this study.

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Peng, B., Hu, S., Jun, Q. et al. MicroRNA-200b targets CREB1 and suppresses cell growth in human malignant glioma. Mol Cell Biochem 379, 51–58 (2013). https://doi.org/10.1007/s11010-013-1626-6

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  • DOI: https://doi.org/10.1007/s11010-013-1626-6

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