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Differential effects of mesenchymal stem cells on a heterogeneous cell population within lung cancer cell lines

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Abstract

Although mesenchymal stem cells (MSCs) promote lung cancer growth in vivo, in vitro studies indicate that they inhibit the proliferation of lung cancer cells. Because malignant tumors contain a heterogeneous cell population with variable capacity for self-renewal, the aim of this study was to determine whether the inconsistencies between in vitro and in vivo studies are a result of differential effects of MSCs on the heterogeneous cell population within lung cancer cell lines. Human MSCs were isolated from the bone marrow, and their cell surface antigen expression and multi-lineage differentiation capacity was examined at passage 10. CD133+ cells were isolated from A549 and H446 cell lines using immunomagnetic separation. The effects of MSCs on the growth and microsphere formation of heterogeneous cell populations within two lung cancer cell lines (A549 and H446) were compared. MSCs inhibited the in vitro proliferation of both cell lines, but significantly accelerated tumor formation and stimulated tumor growth in vivo (P < 0.05). In CD133+ cells isolated from both A549 and H446 cells, co-culture with MSCs for 1–3 days significantly increased their proliferation (P < 0.05). MSCs also significantly increased microsphere formation in both cell lines (P < 0.05). Selective stimulation of CD133+ cell growth may account for the discrepant effects of MSCs on lung cancer progression.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (30871123, 81071728).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Respiratory, Southwest Hospital, Third Military Medical University of PLA, Gaotanyan Street 30, Shapingba District, Chongqing, 400038, China

    Dan Luo, Xiuping Yan, Xiangdong Zhou & Guoxiang Liu

  2. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

    Dengqun Liu

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  1. Dan Luo
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  2. Xiuping Yan
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  3. Dengqun Liu
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  4. Xiangdong Zhou
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Correspondence to Guoxiang Liu.

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Luo, D., Yan, X., Liu, D. et al. Differential effects of mesenchymal stem cells on a heterogeneous cell population within lung cancer cell lines. Mol Cell Biochem 378, 107–116 (2013). https://doi.org/10.1007/s11010-013-1600-3

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  • DOI: https://doi.org/10.1007/s11010-013-1600-3

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