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Biochemical markers of vascular calcification in elderly hemodialysis patients

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Abstract

The increased vascular calcification, cardiovascular morbidity, and mortality in chronic kidney disease (CKD) patients has been associated with disturbances in mineral-bone metabolism. In order to determine markers of the vascular calcification frequently observed in these patients, blood samples of elderly male and female hemodialysis CKD patients were used to measure serum levels of: osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-κB ligand (sRANKL), and fetuin-A by enzyme immunoassay; tartrate-resistant acid phosphatase (TRACP-5b), and bone-specific alkaline phosphatase (BAP) by immunoenzymometric assay; osteocalcin (OC) by ELISA; iPTH by immunoradiometric assay; 25(OH)D3 and 1,25(OH)2D3, by I125 radioimmunoassay; and calcium and phosphorus by photometric assay. Serum OPG, BAP, iPTH, phosphorus, and OC levels were higher and serum 25(OH)D3, 1,25(OH)2D3, and fetuin-A levels lower in both male and female CKD patients than in their respective controls. Our results indicate that the bone formation and resorption parameters are altered in elderly male and female hemodialysis CKD patients. These changes may lead to vascular calcifications and cardiovascular complications, given that elevated OPG and OC levels and reduced fetuin-A levels are associated with cardiovascular events.

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Acknowledgments

The authors thank Magdalena Quintana for technical assistance and Richard Davies for revising the English text. This study was funded in part by grant CICYT/FEDER (BFU2008-06843) and by the Andalusian Regional Government (Endocrinology & Metabolism Group).

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Authors and Affiliations

  1. Vascular Surgery Unit, Sanitas Hospital, Madrid, Spain

    Alvaro Osorio

  2. Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid s/n, 18012, Granada, Spain

    Esperanza Ortega, Jesús M. Torres, Pilar Sanchez & Estrella Ruiz-Requena

  3. Institute of Neurosciences, University of Granada, Granada, Spain

    Esperanza Ortega & Jesús M. Torres

Authors
  1. Alvaro Osorio
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  2. Esperanza Ortega
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  3. Jesús M. Torres
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  4. Pilar Sanchez
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  5. Estrella Ruiz-Requena
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Correspondence to Esperanza Ortega.

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Osorio, A., Ortega, E., Torres, J.M. et al. Biochemical markers of vascular calcification in elderly hemodialysis patients. Mol Cell Biochem 374, 21–27 (2013). https://doi.org/10.1007/s11010-012-1500-y

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  • DOI: https://doi.org/10.1007/s11010-012-1500-y

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