Abstract
The aim of present work was to elucidate the role of actin-depolymerizing factor (ADF), an important regulator of actin cytoskeleton, in the oxidized low-density lipoprotein (ox-LDL)-induced blood–brain barrier (BBB) disruption. The primary mouse brain microvascular endothelial cells (MBMECs) were exposed to ox-LDL. Treatment with LDL served as control. It was found that ADF mRNA level and protein expression were decreased when exposed to ox-LDL in MBMECs. Then, we investigated the influence of ADF overexpression on ox-LDL-treated MBMECs. Structurally, overexpression of ADF inhibited ox-LDL-induced F-actin formation. Functionally, overexpression of ADF attenuated ox-LDL-induced disruption of endothelial barrier marked by restoration of transendothelial electrical resistance, permeability of Evans Blue and expression of tight junction-associated proteins including ZO-1 and occludin, and blocked ox-LDL-induced oxidative stress marked by inhibition of reactive oxygen species (ROS) formation and activity of NADPH oxidase and Nox2 expression. However, overexpression of ADF in control cells had no significant effect on endothelial permeability and ROS formation. In conclusion, overexpression of ADF blocks ox-LDL-induced disruption of endothelial barrier. In addition, siRNA-mediated downregulation of ADF expression aggravated ox-LDL-induced disruption of endothelial barrier and ROS formation. These findings identify ADF as a key signaling molecule in the regulation of BBB integrity and suggest that ADF might be used as a target to modulate diseases accompanied by ox-LDL-induced BBB compromise.
Similar content being viewed by others
References
Engelhardt B (2003) Development of the blood–brain barrier. Cell Tissue Res 314:119–129
Kalayci R, Kaya M, Uzun H, Bilgic B, Ahishali B, Arican N, Elmas I, Küçük M (2009) Influence of hypercholesterolemia and hypertension on the integrity of the blood–brain barrier in rats. Int J Neurosci 119:1881–1904
Nag S, Robertson DM, Dinsdale HB (1980) Morphological changes in spontaneously hypertensive rats. Acta Neuropathol 52:27–34
Min LJ, Mogi M, Shudou M, Jing F, Tsukuda K, Ohshima K, Iwanami J, Horiuchi M (2012) Peroxisome proliferator-activated receptor-γ activation with angiotensin II Type 1 receptor blockade is pivotal for the prevention of blood–brain barrier impairment and cognitive decline in Type 2 diabetic mice. Hypertension 59:1079–1088
Yla-Herttuala S, Palinski W, Rosenfeld ME, Parthasarathy S, Carew TE, Butler S, Witztum JL, Steinberg D (1989) Evidence for the presence of oxidatively modified low density lipoprotein in atherosclerotic lesions of rabbit and man. J Clin Investig 84:1086–1095
Chen TG, Chen TL, Chang HC, Tai YT, Cherng YG, Chang YT, Chen RM (2007) Oxidized low-density lipoprotein induces apoptotic insults to mouse cerebral endothelial cells via a Bax-mitochondria-caspase protease pathway. Toxicol Appl Pharmacol 219:42–53
Kim JH, Lee SJ, Kim KW, Yu YS, Kim JH (2012) Oxidized low density lipoprotein-induced senescence of retinal pigment epithelial cells is followed by outer blood–retinal barrier dysfunction. Int J Biochem Cell Biol 44:808–814
Lin YL, Chang HC, Chen TL, Chang JH, Chiu WT, Lin JW, Chen RM (2010) Resveratrol protects against oxidized LDL-induced breakage of the blood–brain barrier by lessening disruption of tight junctions and apoptotic insults to mouse cerebrovascular endothelial cells. J Nutr 140:2187–2192
Nag S (1995) Role of the endothelial cytoskeleton in blood–brain-barrier permeability to protein. Acta Neuropathol 90:454–460
Prasaina N, Stevensa T (2009) The actin cytoskeleton in endothelial cell phenotypes. Microvasc Res 77:53–63
Essler M, Retzer M, Bauer M, Heemskerk JW, Aepfelbacher M, Siess W (1999) Mildly oxidized low density lipoprotein induces contraction of human endothelial cells through activation of Rho/Rho kinase and inhibition of myosin light chain phosphatase. J Biol Chem 274:30361–30364
Kowalsky GB, Byfield FJ, Levitan I (2008) oxLDL facilitates flow-induced realignment of aortic endothelial cells. Am J Physiol Cell Physiol 295:C332–C340
Serezani CH, Kane S, Medeiros AI, Cornett AM, Kim SH, Marques MM, Lee SP, Lewis C, Bourdonnay E, Ballinger MN, White ES, Peters-Golden M (2012) PTEN directly activates the actin depolymerization factor cofilin-1 during PGE2-mediated inhibition of phagocytosis of fungi. Sci Signal 5:ra12
Galkin VE, Orlova A, Kudryashov DS, Solodukhin A, Reisler E, Schröder GF, Egelman EH (2011) Remodeling of actin filaments by ADF/cofilin proteins. Proc Natl Acad Sci USA 108:20568–20572
McGough A, Pope B, Chiu W, Weeds A (1997) Cofilin changes the twist of F-actin: implications for actin filament dynamics and cellular function. J Cell Biol 138:771–781
Carbó C, Arderiu G, Escolar G, Fusté B, Cases A, Carrascal M, Abián J, Díaz-Ricart M (2008) Differential expression of proteins from cultured endothelial cells exposed to uremic versus normal serum. Am J Kidney Dis 51:603–612
Lyck R, Ruderisch N, Moll AG, Steiner O, Cohen CD, Engelhardt B, Makrides V, Verrey F (2009) Culture-induced changes in blood–brain barrier transcriptome: implications for amino-acid transporters in vivo. J Cereb Blood Flow Metab 29:1491–1502
Vartiainen MK, Mustonen T, Mattila PK, Ojala PJ, Thesleff I, Partanen J, Lappalainen P (2002) The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. Mol Biol Cell 13:183–194
Rao PV, Maddala R, John F, Zigler JS Jr (2004) Expression of nonphagocytic NADPH oxidase system in the ocular lens. Mol Vis 10:112–121
Dimitrijevic OB, Stamatovic SM, Keep RF, Andjelkovic AV (2006) Effects of the chemokine CCL2 on blood–brain barrier permeability during ischemia reperfusion injury. J Cereb Blood Flow Metab 26:797–810
Eugenin EA, Osiecki K, Lopez L, Goldstein H, Calderon TM, Berman JW (2006) CCL2/monocyte chemoattractant protein-1 mediates enhanced transmigration of human immunodeficiency virus (HIV)-infected leukocytes across the blood–brain barrier: a potential mechanism of HIV-CNS invasion and NeuroAIDS. J Neurosci 26:1098–1106
Verdoni AM, Aoyama N, Ikeda A, Ikeda S (2008) Effect of destrin mutations on the gene expression profile in vivo. Physiol Genomics 34:9–21
Gardner GC, Banka L, Roberts KA, Mullick AE, Rutledge JC (1999) Modified LDL-mediated increases in endothelial layer permeability are attenuated with 17b-estradiol. Arterioscler Thromb Vasc Biol 19:854–861
Byfield FJ, Tikku S, Rothblat GH, Gooch KJ, Levitan I (2006) OxLDL increases endothelial stiffness, force generation, and network formation. J Lipid Res 47:715–723
Wang DY, Yang VC, Chen JK (1997) Oxidized LDL inhibits vascular endothelial cell morphogenesis in culture. In Vitro Cell Dev Biol Anim 33:248–255
Miller YI, Viriyakosol S, Binder CJ, Feramisco JR, Kirkland TN, Witztum JL (2003) Minimally modified LDL binds to CD14, induces macrophage spreading via TLR4/MD-2, and inhibits phagocytosis of apoptotic cells. J Biol Chem 278:1561–1568
Miller YI, Worrall DS, Funk CD, Feramisco JR, Witztum JL (2003) Actin polymerization in macrophages in response to oxidized LDL and apoptotic cells: role of 12/15-lipoxygenase and phosphoinositide 3-kinase. Mol Biol Cell 14:4196–4206
Mine S, Tabata T, Wada Y, Fujisaki T, Iida T, Noguchi N, Niki E, Kodama T, Tanaka Y (2002) Oxidized low density lipoprotein-induced LFA-1-dependent adhesion and transendothelial migration of monocytes via the protein kinase C pathway. Atherosclerosis 160:281–288
Dudek SM, Garcia JG (2001) Cytoskeletal regulation of pulmonary vascular permeability. J Appl Physiol 91:1487–1500
Phillips PG, Lum H, Malik AB, Tsan MF (1989) Phallacidin prevents thrombin-induced increases in endothelial permeability to albumin. Am J Physiol 257:C562–C567
Freeman LR, Keller JN (2012) Oxidative stress and cerebral endothelial cells: regulation of the blood–brain-barrier and antioxidant based interventions. Biochim Biophys Acta 1822:822–829
Kahles T, Luedike P, Endres M, Galla HJ, Steinmetz H, Busse R, Neumann-Haefelin T, Brandes RP (2007) NADPH oxidase plays a central role in blood–brain barrier damage in experimental stroke. Stroke 38:3000–3006
Hotulainen P, Paunola E, Vartiainen MK, Lappalainen P (2005) Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. Mol Biol Cell 16:649–664
Author information
Authors and Affiliations
Corresponding author
Additional information
Jun Wang and Lu Sun contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Wang, J., Sun, L., Si, YF. et al. Overexpression of actin-depolymerizing factor blocks oxidized low-density lipoprotein-induced mouse brain microvascular endothelial cell barrier dysfunction. Mol Cell Biochem 371, 1–8 (2012). https://doi.org/10.1007/s11010-012-1415-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11010-012-1415-7