Skip to main content

Advertisement

SpringerLink
Log in

Citrate synthase, sarcoplasmic reticular calcium ATPase, and choline acetyltransferase activities of specific pelvic floor muscles of the rabbit

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

There is a clear relationship between the pelvic floor muscles and urinary systems, which relates to urgency, frequency, incontinence, pelvic pain, and bowel complaints. The specific mechanisms which relate these two systems are not clear. Improved understanding of the relation between the pelvic floor muscles and bladder function is clinically relevant in establishing effective treatments to such problems as incontinence, secondary to birth. The following tissues were collected from normal adult female rabbits: pubococcygeus (Pc) and ischiocavernosus/bulbospongiosus (Ic/Bs) pelvic floor muscles. Bladder body muscle and mucosa, bladder base muscle and mucosa, and leg skeletal muscle were also collected. The following enzymatic assays were performed on each tissue: citrate synthase (CS), sarcoplasmic–endoplasmic reticular ATPase (SERCA), and choline acetyltransferase (ChAT). CS and SERCA activities were significantly higher in the Pc compared with the Ic/Bs pelvic floor muscles, whereas the ChAT activity of the Ic/Bs was higher than that of the Pc muscle. Based on our results, the Pc muscle is expected to have a significantly greater capacity to contract and a higher metabolic activity than those of the Ic/Bs muscles. We believe that an understanding of the biochemical activities of these three biomarker enzymes in normal pelvic floor muscles is essential in evaluating the effects of specific experimental dysfunctions created in pelvic floor muscle activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Shafik A, El-Sibai O (2001) Effect of levator ani muscle contraction on urethrovesical and anorectal pressures and role of the muscle in urination and defecation. Urology 58:193–197

    Article  PubMed  CAS  Google Scholar 

  2. Lamvu G, Williams R, Zolnoun D, Wechter ME, Shortliffe A, Fulton G, Steege JF (2006) Long-term outcomes after surgical and nonsurgical management of chronic pelvic pain: one year after evaluation in a pelvic pain specialty clinic. Am J Obstet Gynecol 195:591–598 (discussion 598–600)

    Article  PubMed  Google Scholar 

  3. Dumoulin C, Hay-Smith J (2010) Pelvic floor muscle training versus no treatment, or inactive control treatments, for urinary incontinence in women. Cochrane Database Syst Rev 1: CD005654

  4. Koh CE, Young CJ, Young JM, Solomon MJ (2008) Systematic review of randomized controlled trials of the effectiveness of biofeedback for pelvic floor dysfunction. Br J Surg 95:1079–1087

    Article  PubMed  CAS  Google Scholar 

  5. Wang AC, Wang YY, Chen MC (2004) Single-blind, randomized trial of pelvic floor muscle training, biofeedback-assisted pelvic floor muscle training, and electrical stimulation in the management of overactive bladder. Urology 63:61–66

    Article  PubMed  CAS  Google Scholar 

  6. Whitford HM, Jones M (2011) An exploration of the motivation of pregnant women to perform pelvic floor exercises using the revised theory of planned behaviour. Br J Health Psychol 16:761–778

    Article  PubMed  Google Scholar 

  7. Santacreu M, Fernandez-Ballesteros R (2011) Evaluation of a behavioral treatment for female urinary incontinence. Clin Interv Aging 6:133–139

    Article  PubMed  Google Scholar 

  8. Dumoulin C, Glazener C, Jenkinson D (2011) Determining the optimal pelvic floor muscle training regimen for women with stress urinary incontinence. Neurourol Urodyn 30:746–753

    Article  PubMed  Google Scholar 

  9. Simard C, le Tu M (2010) Long-term efficacy of pelvic floor muscle rehabilitation for older women with urinary incontinence. J Obstet Gynaecol Can 32:1163–1166

    PubMed  Google Scholar 

  10. Hung HC, Hsiao SM, Chih SY, Lin HH, Tsauo JY (2011) Effect of pelvic-floor muscle strengthening on bladder neck mobility: a clinical trial. Phys Ther 91:1030–1038

    Article  PubMed  Google Scholar 

  11. Malykhina AP (2007) Neural mechanisms of pelvic organ cross-sensitization. Neuroscience 149:660–672

    Article  PubMed  CAS  Google Scholar 

  12. Buttyan R, Chen MW, Levin RM (1997) Animal models of bladder outlet obstruction and molecular insights into the basis for the development of bladder dysfunction. Eur Urol 32(Suppl 1):32–39

    PubMed  CAS  Google Scholar 

  13. Levin RM, Haugaard N, Mogavero L, Leggett RE, Das AK (1999) Biochemical evaluation of obstructive bladder dysfunction in men secondary to BPH: a preliminary report. Urology 53:446–450

    Article  PubMed  CAS  Google Scholar 

  14. Levin RM, Haugaard N, O’Connor L, Buttyan R, Das A, Dixon JS, Gosling JA (2000) Obstructive response of human bladder to BPH vs. rabbit bladder response to partial outlet obstruction: a direct comparison. Neurourol Urodyn 19:609–629

    Article  PubMed  CAS  Google Scholar 

  15. Kato K, Monson FC, Longhurst PA, Wein AJ, Haugaard N, Levin RM (1990) The functional effects of long-term outlet obstruction on the rabbit urinary bladder. J Urol 143:600–606

    PubMed  CAS  Google Scholar 

  16. Cruz Y, Hudson R, Pacheco P, Lucio RA, Martinez-Gomez M (2002) Anatomical and physiological characteristics of perineal muscles in the female rabbit. Physiol Behav 75:33–40

    Article  PubMed  CAS  Google Scholar 

  17. Corona-Quintanilla DL, Castelan F, Fajardo V, Manzo J, Martinez-Gomez M (2009) Temporal coordination of pelvic and perineal striated muscle activity during micturition in female rabbits. J Urol 181:1452–1458

    Article  PubMed  Google Scholar 

  18. Bouzidi MF, Enjolras N, Carrier H, Vial C, Lopez-Mediavilla C, Burt-Pichat B, Couthon F, Godinot C (1996) Variations of muscle mitochondrial creatine kinase activity in mitochondrial diseases. Biochim Biophys Acta 1316:61–70

    Article  PubMed  Google Scholar 

  19. Sherratt HS (1991) Mitochondria: structure and function. Rev Neurol (Paris) 147:417–430

    CAS  Google Scholar 

  20. Beeckmans S (1984) Some structural and regulatory aspects of citrate synthase. Int J Biochem 16:341–351

    Article  PubMed  CAS  Google Scholar 

  21. Levin RM, Nicholas TJ, Snitkoff GG, Mandell J, Russell D, Wilbur HJ, Mogavero LJ (1997) Subcellular distribution of SERCA and calcium-activated ATPase in rabbit and human urinary bladder smooth muscle. Pharmacology 55:309–316

    Article  PubMed  CAS  Google Scholar 

  22. Moller JV, Olesen C, Winther AM, Nissen P (2010) The sarcoplasmic Ca2+-ATPase: design of a perfect chemi-osmotic pump. Q Rev Biophys 43:501–566

    Article  PubMed  Google Scholar 

  23. Brini M, Carafoli E (2009) Calcium pumps in health and disease. Physiol Rev 89:1341–1378

    Article  PubMed  CAS  Google Scholar 

  24. Contestabile A, Ciani E (2008) The place of choline acetyltransferase activity measurement in the “cholinergic hypothesis” of neurodegenerative diseases. Neurochem Res 33:318–327

    Article  PubMed  CAS  Google Scholar 

  25. Dobransky T, Rylett RJ (2003) Functional regulation of choline acetyltransferase by phosphorylation. Neurochem Res 28:537–542

    Article  PubMed  CAS  Google Scholar 

  26. Haugaard N, Potter L, Wein AJ, Levin RM (1992) Effect of partial obstruction of the rabbit urinary bladder on malate dehydrogenase and citrate synthase activity. J Urol 147:1391–1393

    PubMed  CAS  Google Scholar 

  27. Levin RM, Saito M, Wein AJ, Packard D, Cohen A, Haugaard N (1993) Effect of partial outlet obstruction on choline acetyltransferase activity in the rat and rabbit. Neurourol Urodyn 12:255–261

    Article  PubMed  CAS  Google Scholar 

  28. Hypolite JA, Longhurst PA, Gong C, Briscoe J, Wein AJ, Levin RM (1993) Metabolic studies on rabbit bladder smooth muscle and mucosa. Mol Cell Biochem 125:35–42

    Article  PubMed  CAS  Google Scholar 

  29. Levin RM, Haugaard N, Levin SS, Buttyan R, Chen MW, Monson FC, Wein AJ (1995) Bladder function in experimental outlet obstruction: pharmacologic responses to alterations in innervation, energetics, calcium mobilization, and genetics. Adv Exp Med Biol 385:7–19 (discussion 75–79)

    PubMed  CAS  Google Scholar 

  30. Levin RM, Longhurst PA, Monson FC, Kato K, Wein AJ (1990) Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder. Prostate Suppl 3:9–26

    Article  PubMed  CAS  Google Scholar 

  31. Bupha-Intr T, Laosiripisan J, Wattanapermpool J (2009) Moderate intensity of regular exercise improves cardiac SR Ca2+ uptake activity in ovariectomized rats. J Appl Physiol 107:1105–1112

    Article  PubMed  CAS  Google Scholar 

  32. Kemi OJ, Ceci M, Condorelli G, Smith GL, Wisloff U (2008) Myocardial sarcoplasmic reticulum Ca2+ ATPase function is increased by aerobic interval training. Eur J Cardiovasc Prev Rehabil 15:145–148

    Article  PubMed  Google Scholar 

  33. Levin RM, Haugaard N, Wein AJ (1992) Metabolic alterations induced by obstructive hypertrophy of the rabbit urinary bladder. Jpn J Pharmacol 58(Suppl 2):341P

    PubMed  Google Scholar 

Download references

Acknowledgments

This material is based upon work supported in part by the Office of Research and Development Medical Research Service, Department of Veterans Affairs, and in part, by the Capital Region Medical Research Foundation.

Conflict of interest

No financial conflicts of interest are involved with any of the authors.

Author information

Authors and Affiliations

  1. Stratton VA Medical Center, Albany, NY, 12208, USA

    Catherine Schuler, Robert E. Leggett & Robert M. Levin

  2. Albany College of Pharmacy and Health Sciences, Albany, NY, 12208, USA

    Tamer Elias & Robert M. Levin

  3. Albany Medical Center, Albany, NY, 12208, USA

    Robert M. Levin

  4. Urology, Albany Medical Center, Albany, NY, USA

    Sara Spettel & Elise De

Authors
  1. Sara Spettel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elise De
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tamer Elias
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Catherine Schuler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Robert E. Leggett
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Robert M. Levin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Robert M. Levin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spettel, S., De, E., Elias, T. et al. Citrate synthase, sarcoplasmic reticular calcium ATPase, and choline acetyltransferase activities of specific pelvic floor muscles of the rabbit. Mol Cell Biochem 370, 1–5 (2012). https://doi.org/10.1007/s11010-012-1347-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11010-012-1347-2

Keywords

Search

Navigation