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Effect of hepcidin on intracellular calcium in human osteoblasts

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Abstract

Hepcidin is known to increase intracellular iron through binding to and degrading ferroportin, which is a transmembrane protein that transports iron from the intracellular to the outside. However, it is not clear whether hepcidin has a similar effect on intracellular calcium. Here, we investigated the influence of hepcidin on intracellular calcium in human osteoblasts, with or without high environmental iron concentrations. Our data showed that hepcidin (<100 nmol/L) could increase intracellular calcium, and this effect was more significant when cells were exposed to high environmental iron concentrations. To further explore its underlying mechanisms, we pretreated human osteoblasts with Nimodipine, a L-type calcium channel blocker, and Dantrolene, a ryanodine receptor antagonist to inhibit abnormal calcium release from the sarco-endoplasmic reticulum. These treatments had not resulted in any alteration of intracellular calcium in human osteoblasts. Thus, these findings indicate that the increase of intracellular calcium induced by hepcidin is probably due to calcium release from endoplasmic reticulum, which is triggered by calcium influx.

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Acknowledgments

This research is supported by Natural Science Foundation of Jiangsu Province (BK2008165), Programs Foundation of Ministry of Education of China (20103201110020) and Postgraduate Innovation Fund of Jiangsu Province (CX10B-053Z).

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Authors and Affiliations

  1. Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, Jiangsu, China

    Guang-Fei Li, You-Jia Xu, Yin-Feng He, Ben-Cai Du, Peng Zhang, Dong-Yang Zhao & Chen Yu

  2. Department of Docimasiology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, Jiangsu, China

    Chen-Hao Qin

  3. Department of Molecular Diagnostics and Biopharmaceutics, College of Pharmacy, Soochow University, Suzhou, 215123, Jiangsu, China

    Kai Li

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  1. Guang-Fei Li
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  8. Chen-Hao Qin
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  9. Kai Li
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Correspondence to You-Jia Xu.

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Li, GF., Xu, YJ., He, YF. et al. Effect of hepcidin on intracellular calcium in human osteoblasts. Mol Cell Biochem 366, 169–174 (2012). https://doi.org/10.1007/s11010-012-1294-y

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  • DOI: https://doi.org/10.1007/s11010-012-1294-y

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