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Involvement of ecto-5′-nucleotidase/CD73 in U138MG glioma cell adhesion

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Abstract

Glioblastoma multiform is the most common and aggressive type of brain tumor. The overexpression of ecto-5′-nucleotidase/CD73 (ecto-5′-NT/CD73), an adhesion molecule and the main enzymatic source of extracellular adenosine, has been reported in tumor cells, and it is emerging as a component of glioma progression. Here, we evaluated the involvement of ecto-5′-NT/CD73 in cell adhesion through its interaction with different components of the extracellular matrix in the human U138MG glioma cell line. The results indicated that adenosine induced an increase in glioma cell adhesion. The treatment of glioma cells with adenosine receptor antagonists, APCP (α,β-methylene ADP) and dipyridamole prevented the adenosine effect, indicating the participation of extracellular and intracellular signaling pathways in cell adhesion mediated by adenosine. The ECM protein laminin (lam) and chondroitin sulfate (ChS) modulated the ecto-5′-NT/CD73 activity and glioma adhesion in a parallel manner, suggesting the involvement of purinergic signaling in the effects mediated by the extracellular matrix. Taken together, these results suggest that ecto-5′-NT/CD73, an important producer of extracellular adenosine, may modulate glioma cell adhesion and tumor cell–extracellular matrix interactions.

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Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). A.R. Cappellari, L. Bavaresco and E. Braganhol were recipients of Capes fellowships and G.J. Vasques was a recipient of a FAPERGS fellowship. We thank L.R. Blazina for her technical assistance.

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  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-anexo, Porto Alegre, RS, 90035-003, Brazil

    Angélica Regina Cappellari, Gabriela Jouglard Vasques, Luci Bavaresco, Elizandra Braganhol & Ana Maria Oliveira Battastini

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  1. Angélica Regina Cappellari
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  2. Gabriela Jouglard Vasques
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  3. Luci Bavaresco
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  5. Ana Maria Oliveira Battastini
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Correspondence to Ana Maria Oliveira Battastini.

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Cappellari, A.R., Vasques, G.J., Bavaresco, L. et al. Involvement of ecto-5′-nucleotidase/CD73 in U138MG glioma cell adhesion. Mol Cell Biochem 359, 315–322 (2012). https://doi.org/10.1007/s11010-011-1025-9

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  • DOI: https://doi.org/10.1007/s11010-011-1025-9

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