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Perifosine as potential anti-cancer agent inhibits proliferation, migration, and tube formation of human umbilical vein endothelial cells

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Abstract

Targeting angiogenesis is considered an effective strategy for treating the expansion and metastasis of tumors. The aim of this study is to assess the effects of perifosine, an inhibitor of Akt, on cell proliferation, apoptosis, angiogenesis, and VEGF-induced cell migration in cultured human umbilical vein endothelial cells (HUVECs) in vitro. MTT and cell cycle analysis results indicated that perifosine inhibited the growth of HUVECs in a dose-dependent manner, arrested cell cycle progression at the G2 phase with regulation the expression of p21 and cyclinB1. Apoptosis induced by the higher concentrations of perifosine in HUVECs was also observed. In addition, tube formation of HUVECs and VEGF-induced cell migration were markedly inhibited by perifosine. Western blotting analysis of cell signaling molecules indicated that perifosine inhibited ERK and p38 phosphorylation in HUVECs. These results suggest that perifosine exerts anti-angiogenic activity in HUVECs and is a promising agent for treatment of angiogenesis related-diseases.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (No 30800422).

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Authors and Affiliations

  1. School of Biological Science, Taishan Medical University, Chang Cheng Road, Taian, 271016, People’s Republic of China

    Feng Ze Wang, Xiao Qian Li, Renjiu Shi & De Cai Wang

  2. Institute of Medicinal Biotechnology, Taishan Medical University, Chang Cheng Road, Taian, 271016, People’s Republic of China

    Feng Ze Wang & Renjiu Shi

  3. School of Pharmacy, Taishan Medical University, Chang Cheng Road, Taian, 271016, People’s Republic of China

    Hong Rong Fei

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  1. Feng Ze Wang
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  2. Hong Rong Fei
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  3. Xiao Qian Li
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Correspondence to Feng Ze Wang.

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Wang, F.Z., Fei, H.R., Li, X.Q. et al. Perifosine as potential anti-cancer agent inhibits proliferation, migration, and tube formation of human umbilical vein endothelial cells. Mol Cell Biochem 368, 1–8 (2012). https://doi.org/10.1007/s11010-011-0986-z

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  • DOI: https://doi.org/10.1007/s11010-011-0986-z

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