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Differential expression of oncogenic miRNAs in proliferating and senescent human fibroblasts

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Abstract

miRNAs are a class of non-coding RNAs that play fundamental roles through the post-transcriptional regulation of target mRNAs. miRNAs have been shown to regulate a broad spectrum of biological activities, including development, differentiation, cell death, and oncogenesis. However, little is known about their contribution to cellular senescence. The authors analyzed the expression of 576 miRNAs in proliferating and senescent normal human fibroblasts by microarray, and identified 12 miRNAs that were differentially expressed in proliferating and senescent fibroblasts. Interestingly, all six miRNAs that were down-regulated in senescent cells had been previously reported to be aberrantly expressed in tumor cells. It was further showed that inhibition of miR-17-5p and miR-20a by 2′-O-methyl antisense oligoribonucleotides resulted in the induction of senescent phenotypes in WI-38 cells.

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Acknowledgments

This study was supported by the grants from the “973” Project of the Ministry of Science and Technology [2007CB507402, 2007CB914402, 2010CB912802] and the grants from the National Natural Science Foundation of China [30671065, 30871258].

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Authors and Affiliations

  1. Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, 19 Xin Jie Kou Wai Avenue, Beijing, 100875, China

    Miao Wang, Zhaojie Cheng, Tian Tian, Jingwen Chen, Fei Dou & Yu-Sheng Cong

  2. Department of Gastroenterology & Hepatology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China

    Mingzhou Guo

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  1. Miao Wang
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  2. Zhaojie Cheng
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  3. Tian Tian
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  7. Yu-Sheng Cong
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Correspondence to Yu-Sheng Cong.

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11010_2011_763_MOESM1_ESM.jpg

Expression profile of selected miRNAs in young replicating, senescent and quiescent cells by stem-loop qRT–PCR. Relative expression levels of the miRNAs (miR-15b, miR-17-5p, miR-20a, miR-25, miR-93, miR-106a, miR-127-3p, mir-329, miR-409-3p, miR-487b, miR-493*, miR-495) identified by microarray were analyzed by stem-loop qRT–PCR in different growth states of WI-38 cells. U6 RNA was used for normalization. Data are presented as the mean ± SD from three independent experiments. (JPEG 137 kb)

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Wang, M., Cheng, Z., Tian, T. et al. Differential expression of oncogenic miRNAs in proliferating and senescent human fibroblasts. Mol Cell Biochem 352, 271–279 (2011). https://doi.org/10.1007/s11010-011-0763-z

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  • DOI: https://doi.org/10.1007/s11010-011-0763-z

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