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The influence of training status on oxidative stress in young male handball players

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Abstract

Although exercise-induced oxidative stress receives considerable scientific attention, there is still little information available regarding exercise-induced adaptations of the antioxidant defence system in adolescent and child athletes. The aim of our study was to establish the effects of long-term exercise training on the redox state of adolescents, and to find correlations between elements of redox homeostasis and aerobic power. Thirty-three handball players and 14 non-athletes, 16–19-years old, were subjected to blood sampling to measure levels of nitric oxide (NO; estimated through nitrites (NO2 –)), superoxide anion radical (O2 –), hydrogen peroxide (H2O2), lipid peroxidation (estimated through TBARS), superoxide dismutase (SOD) and catalase (CAT). Subjects were also subjected to maximal progressive exercise test to estimate their maximal oxygen consumption (\( {{{\text{VO}}_{ 2} { \max }}} \)). Athletes had significantly (P < 0.05) higher SOD activity and lower CAT activity compared with non-athletes (SOD: 2175.52 ± 362.07 compared with 1172.16 ± 747.40 U/g of hemoglobin × 10 3, and CAT: 2.19 ± 0.31 compared with 3.08 ± 0.47 U/g of hemoglobin × 103). These differences were the most obvious when comparing non-athletes and athletes with poor/average aerobic power. H2O2 and TBARS levels differed among subjects with poor, average or good aerobic power (P < 0.01, and P < 0.05, respectively). Sports engagament and aerobic capacity are important factors in inducing changes in redox status.

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Acknowledgments

This work was supported by Grant No. 175043 from the Ministry of Science and Technical Development of the Republic of Serbia.

Conflict of interest

The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Kragujevac, Svetozara Markovica 69, P.P. 124, 34000, Kragujevac, Republic of Serbia

    Dusica Djordjevic, Dejan Cubrilo, Nevena Barudzic & Vladimir Jakovljevic

  2. Faculty of Sports and Physical Education, University of Belgrade, Belgrade, Republic of Serbia

    Marija Macura

  3. Institute of Medical Physiology “Richard Burian”, School of Medicine, Belgrade, Republic of Serbia

    Dragan Djuric

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  1. Dusica Djordjevic
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  2. Dejan Cubrilo
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  3. Marija Macura
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  4. Nevena Barudzic
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  5. Dragan Djuric
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  6. Vladimir Jakovljevic
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Correspondence to Vladimir Jakovljevic.

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Djordjevic, D., Cubrilo, D., Macura, M. et al. The influence of training status on oxidative stress in young male handball players. Mol Cell Biochem 351, 251–259 (2011). https://doi.org/10.1007/s11010-011-0732-6

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