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Globular adiponectin regulates energy homeostasis through AMP-activated protein kinase–acetyl-CoA carboxylase (AMPK/ACC) pathway in the hypothalamus

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Abstract

Adiponectin is a newly researched adipokine which participates in the regulation of energy homeostasis. AMP-activated protein kinase (AMPK) represents an energy sensor that responds to hormone and nutrition status in vivo and exerts a regulatory effect in the hypothalamus and multiple peripheral tissues. We investigated the possible mechanisms involved in appetite regulation by adiponectin in vitro with GT1-7 cells, a mouse immortalized hypothalamic neuron. The results showed that adiponectin increased the phosphorylation of AMPK, activated AMPK phosphorylated and inactivated acetyl-CoA carboxylase (ACC), and subsequently increased expression of agouti-related peptide (AgRP) mRNA. Our results also indicated that adiponectin had no effect on signal transducer and activator of transcription (STAT3). Together these findings suggest that adiponectin regulated energy homeostasis through the AMPK/ACC pathway but not the JAK/STAT3 pathway in the hypothalamus.

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Acknowledgments

This study was supported by the grants from Natural Science Foundation of China (No.30900510) and Fujian Youth Talent Project (2008F3032).

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Authors and Affiliations

  1. Department of Endocrinology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, China

    Jun-Ping Wen, Chun-e Liu, Ya-Ting Hu, Gang Chen & Li-xiang Lin

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  1. Jun-Ping Wen
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  2. Chun-e Liu
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  3. Ya-Ting Hu
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  4. Gang Chen
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  5. Li-xiang Lin
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Corresponding authors

Correspondence to Gang Chen or Li-xiang Lin.

Additional information

Jun-Ping Wen and Chun-e Liu are equally contributed to this study.

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Wen, JP., Liu, Ce., Hu, YT. et al. Globular adiponectin regulates energy homeostasis through AMP-activated protein kinase–acetyl-CoA carboxylase (AMPK/ACC) pathway in the hypothalamus. Mol Cell Biochem 344, 109–115 (2010). https://doi.org/10.1007/s11010-010-0534-2

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  • DOI: https://doi.org/10.1007/s11010-010-0534-2

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