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Spontaneous skin damage and delayed wound healing in SOD1-deficient mice

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Abstract

Superoxide dismutase 1 (SOD1) is an important antioxidative enzyme that protects skin from oxidative stress. SOD1 −/− mice with a genetic background of b129Sv mice showed facial skin damage after 15 weeks of age. Eyelid swelling occurred as the initial symptom and caused impairment by triggering self-scratching. The period required for wound healing in the back was markedly delayed in 20-week SOD1 −/− mice. Oxidative stress markers, 4-hydroxynonenal and thiobarbituric acid-reactive substances, were unexpectedly lower in SOD1 −/− mice at day 1 after wounding. The decay rate of electron paramagnetic resonance signal intensity of intravenously injected nitroxide radical indicated that the half-life of the signal intensity was significantly prolonged in the wounded skin of SOD1 +/+ mice. However, while the half-life of the signal intensity in control skin was a little longer in SOD1 −/− mice, it did not change in wounded skin. Taken together, these data suggest that the skin of SOD1 −/− mice is in redox imbalance and prone to damage by wounding.

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Acknowledgments

This work was supported, in part, by Global COE Program (F03) from the Japan Society for the Promotion of Science and by the Cosmetology Research Foundation.

Declaration of interest

The authors have no conflict of interest to declare. The authors are responsible for generating the data and preparing the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University, 2-2-2 Iidanishi, Yamagata, 990-9585, Japan

    Yoshihito Iuchi, Dipa Roy, Futoshi Okada, Noriko Kibe, Satoshi Tsunoda, Saori Suzuki & Junichi Fujii

  2. Respiratory and Cardiovascular Diseases Research Center, Research Institute for Advanced Molecular Epidemiology, Yamagata University, Yamagata, 990-9585, Japan

    Yoshihito Iuchi, Futoshi Okada, Noriko Kibe, Satoshi Tsunoda, Saori Suzuki & Junichi Fujii

  3. Global COE Program for Medical Sciences, Japan Society for the Promotion of Science, Yamagata, 990-9585, Japan

    Yoshihito Iuchi, Futoshi Okada, Noriko Kibe, Satoshi Tsunoda, Saori Suzuki & Junichi Fujii

  4. Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan

    Motoko Takahashi

  5. Department of Pharmaceutical Sciences, International University of Health and Welfare, Ohtawara, Tochigi, 324-8501, Japan

    Hidekatsu Yokoyama

  6. Department of Infectious Disease, Faculty of Medicine, Oita University, Yufu, Oita, 879-5593, Japan

    Jun Yoshitake

  7. Kondo Skin Clinic, Tokyo, 184-0011, Japan

    Seiji Kondo

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  1. Yoshihito Iuchi
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  2. Dipa Roy
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  11. Junichi Fujii
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Correspondence to Junichi Fujii.

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Iuchi, Y., Roy, D., Okada, F. et al. Spontaneous skin damage and delayed wound healing in SOD1-deficient mice. Mol Cell Biochem 341, 181–194 (2010). https://doi.org/10.1007/s11010-010-0449-y

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