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Newly identified tumor-associated role of human Sharpin

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Abstract

In order to discover previously unidentified cancer-associated genes, we analyzed genome-wide differences in gene expression between tumor biopsies and normal tissues. Among those differentially regulated genes, we identified Sharpin (Shank-associated RH domain-interacting protein) as a commonly up-regulated gene in multiple human cancer types. Although rat Sharpin is reported to interact with Shank1, a multidomain scaffold protein localized in postsynaptic densities, its exact roles are unknown. Whereas human Sharpin homologue was primarily localized in the cytosol of cultured cells, they were detected in both cytosol and nucleus of the cells from ovarian and liver cancer tissues using immunohistochemical staining. In addition, Chinese ovary hamster cells over-expressing Sharpin exhibited enhanced cancer-specific phenotypes in multiple in vitro tumor assays. Taken together, the results suggest that Sharpin is not an inert scaffold protein, but may play tumor-associated roles during cancer biogenesis.

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Acknowledgments

This study was supported by the grants, 21C Frontier Functional Human Genome Project (FG09-21-16) and Basic Science Research Program (2009-0067522) by National Research Foundation, and Korea Research Foundation Grant (KRF-2006-331-C00268) funded by Korean Government.

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Authors and Affiliations

  1. Department of Microbiology, Chungnam National University, Daejeon, 305-764, Korea

    Jinyoung Jung, Byoungwoo Park, Yeongmi Cheon & Soojin Lee

  2. Department of Pathology and Cancer Research Institute, College of Medicine, Chungnam National University, Daejeon, Korea

    Jin Man Kim

  3. R&D Park, LG Life Sciences, Ltd, Moonji-dong, Daejeon, Korea

    Bogman Lee & Seung Ho Choo

  4. Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea

    Sang Seok Koh

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  1. Jinyoung Jung
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  2. Jin Man Kim
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  3. Byoungwoo Park
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  4. Yeongmi Cheon
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  5. Bogman Lee
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  6. Seung Ho Choo
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  8. Soojin Lee
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Correspondence to Soojin Lee.

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Jung, J., Kim, J.M., Park, B. et al. Newly identified tumor-associated role of human Sharpin . Mol Cell Biochem 340, 161–167 (2010). https://doi.org/10.1007/s11010-010-0413-x

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  • DOI: https://doi.org/10.1007/s11010-010-0413-x

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