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The structure of the 5′-untranslated region of mammalian poly(A) polymerase-α mRNA suggests a mechanism of translational regulation

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Abstract

Poly(A)polymerase-α (PAPOLA) has been the most extensively investigated mammalian polyadenylating enzyme, mainly in regard to its multifaceted post-translational regulation. The possibility of translational regulation of this enzyme was addressed. The transcription start site was mapped and two uORFs, highly conserved among several species, were identified in the 211-bp long, GC-rich, 5′ UTR of the PAPOLA mRNA. Mutation of the 5′ proximal AUG resulted in increased translational efficiency of the adjacent coding sequence, whereas no significant effect was observed after mutation of the second AUG. These observations imply that translational regulation is among the conserved mechanisms regulating PAPOLA expression.

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Authors and Affiliations

  1. Papanikolaou Research Center of Oncology, St Savvas Hospital, Athens, Greece

    Aikaterini Rapti, Panayotis Ioannidis, Euthymios Dimitriadis, Christos Meristoudis & Nelly Courtis

  2. Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

    Theoni Trangas

  3. Biomedical Sciences Research Centre Alexander Fleming, Vari, Greece

    Martina Samiotaki

  4. Microbiology Department, National Reference Center for Mycobacteria, ‘Sotiria’ General Hospital, Athens, Greece

    Panayotis Ioannidis

  5. Department of Biology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece

    Aikaterini Rapti & Stavroula Veletza

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  1. Aikaterini Rapti
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  2. Theoni Trangas
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  3. Martina Samiotaki
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  4. Panayotis Ioannidis
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Correspondence to Theoni Trangas.

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Rapti, A., Trangas, T., Samiotaki, M. et al. The structure of the 5′-untranslated region of mammalian poly(A) polymerase-α mRNA suggests a mechanism of translational regulation. Mol Cell Biochem 340, 91–96 (2010). https://doi.org/10.1007/s11010-010-0405-x

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  • DOI: https://doi.org/10.1007/s11010-010-0405-x

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