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Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro

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Abstract

The effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-dependent manner, while no obvious effect of catapol on the proliferation of hADSCs was observed. In addition, the cell number of hADSCs treated by 1.5 mM PCA increased in a time-dependent manner. The flow cytometric analysis of DNA content demonstrated the cell cycle progress from the G0/G1 phase to the S phase. Western blot analysis revealed the elevated expression of cyclin D1 in hADSCs induced by PCA treatment. Cyclin D1-siRNA transfection significantly inhibit the promotion of cell proliferation by PCA. Furthermore, the flow cytometric analysis of the cell surface antigens and the multidifferential potential tests of PCA-treated hADSCs showed that the cells retained their functional characteristics of multipotential mesenchymal progenitors. It is concluded that PCA can effectively up-regulate the proliferation of hADSCs.

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Acknowledgments

This work was supported by the grants from the National Natural Science Foundation of China (30670525 and 30700181).

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Authors and Affiliations

  1. Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Environmental and Life Science Building 3-403, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, China

    Han Wang, Tian-Qing Liu, Yan-Xia Zhu, Shui Guan & Xue-Hu Ma

  2. Department of Engineering Science, Oxford University, Oxford, OX1 3PJ, UK

    Zhan-Feng Cui

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  1. Han Wang
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  2. Tian-Qing Liu
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Correspondence to Tian-Qing Liu.

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Wang, H., Liu, TQ., Zhu, YX. et al. Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. Mol Cell Biochem 330, 47–53 (2009). https://doi.org/10.1007/s11010-009-0099-0

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