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Antilipoperoxidative and membrane stabilizing effect of diosgenin, in experimentally induced myocardial infarction

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Altered membrane integrity has been suggested as a major factor in the development of cellular injury during myocardial necrosis. The present study was designed to investigate the effect of diosgenin on lysosomal hydrolases, membrane-bound enzymes, and electrolytes during isoproterenol (ISO)-induced myocardial necrosis in rats. Animals were pretreated with DIOS (80 mg/kg) for a period of 35 days. Myocardial infarction was experimentally induced with ISO (85 mg/kg) twice at 24 h interval. Experimental myocardial infarction was evidenced with marked elevation of creatine kinase-MB (CK-MB) in serum with concomitant increase in lipid peroxidation (plasma thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP)). Activity of lysosomal hydrolases (β-glucuronidase, β-N-acetyl glucosaminidase, β-d-galactosidase, cathepsin D, and acid phosphatase) was found to be increased in serum and heart tissue of ISO-alone treated animals. DIOS (80 mg/kg) pretreated groups showed significant decrease in CK-MB, lipid peroxidation, and lysosomal hydrolases activity. The membrane-bound enzymes such as Ca2+-ATPase and Mg2+-ATPase activity was increased and Na+/K+-ATPase activity was decreased in the heart tissues of ISO-alone treated animals. These enzyme alterations lead to the change in the electrolytes content such as sodium, potassium, and calcium in the heart tissue. However, DIOS (80 mg/kg) pretreatment reversed the membrane-bound enzymes activity and thereby maintained the normal electrolyte concentration. These results suggest the protective action of diosgenin in ISO-induced myocardial infarction. The salubrious effect observed in this study might be due to the antioxidant and membrane stabilizing potential of diosgenin.

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Acknowledgments

The authors are grateful to the Drugs and Pharmaceuticals Division, Dept. of Science and Technology, Govt. of India for providing Junior Research fellowship to the first author and Prof. R.Sethuraman, Vice-chancellor, SASTRA University for his support and encouragement during the course of this work.

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  1. Center for Advanced Research in Indian System of Medicine, SASTRA University, Thirumalaisamudram, Thanjavur, 613402, Tamilnadu, India

    K. S. Jayachandran & G. V. Rajamanickam

  2. Department of Biochemistry, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Porur, 600116, Tamilnadu, India

    Hannah R. Vasanthi

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Correspondence to Hannah R. Vasanthi.

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Jayachandran, K.S., Vasanthi, H.R. & Rajamanickam, G.V. Antilipoperoxidative and membrane stabilizing effect of diosgenin, in experimentally induced myocardial infarction. Mol Cell Biochem 327, 203–210 (2009). https://doi.org/10.1007/s11010-009-0058-9

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