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Effects of N-hexacosanol on nitric oxide synthase system in diabetic rat nephropathy

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Abstract

We attempted to clarify the effects of cyclohexenonic long-chain fatty alcohol (N-hexacosanol) on nitric oxide synthase (NOS) in streptozotocin-induced diabetic nephropathy. After induction of experimental diabetes with streptozotocin, rats were maintained for 8 weeks with or without treatment by N-hexacosanol (8 mg/kg i.p. every day). Urinary albumin excretion, blood chemistry, immunoblot analysis, and real-time polymerase chain reactions (real-time PCR) of endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), and neuronal NOS (nNOS) were investigated. Although N-hexacosanol had no effects on serum glucose or insulin level, it normalized serum creatinine and urinary albumin excretion. N-hexacosanol was found to improve the diabetes-induced alterations in the eNOS, iNOS, and nNOS protein and their mRNA levels. Histologically, N-hexacosanol inhibited the progression to glomerular sclerosis. Our data suggest that N-hexacosanol improves diabetes-induced NOS alterations in the kidney, resulting in the amelioration of diabetic nephropathy.

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Acknowledgments

This study was supported in part by the Morinaga Houshikai Research Foundation. We gratefully acknowledge Dr. Y. Kinoshita and I. Satoh, Division of Molecular Pharmacology, Tottori University, Japan, for their technical assistance.

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Authors and Affiliations

  1. Division of Pediatrics and Perinatology, Tottori University, 36-1 Nishi-machi, Yonago, 683-8504, Tottori, Japan

    Shinichi Okada, Takuya Hanada, Yasuo Kawaba, Atsushi Hayashi & Susumu Kanzaki

  2. Division of Molecular Pharmacology, Tottori University, 86 Nishi-machi, Yonago, Tottori, 683-8503, Japan

    Motoaki Saito, Emi Kazuyama & Keisuke Satoh

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  1. Shinichi Okada
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  2. Motoaki Saito
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  3. Emi Kazuyama
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  4. Takuya Hanada
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Correspondence to Motoaki Saito.

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Okada, S., Saito, M., Kazuyama, E. et al. Effects of N-hexacosanol on nitric oxide synthase system in diabetic rat nephropathy. Mol Cell Biochem 315, 169–177 (2008). https://doi.org/10.1007/s11010-008-9804-7

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