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Sphingosine 1-phosphate differentially regulates proliferation of C2C12 reserve cells and myoblasts

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Abstract

The effect of sphingosine 1-phosphate (S1P) on the proliferative response to low serum was examined in two closely related cell populations, such as C2C12 reserve cells and myoblasts. S1P reduced DNA synthesis promoted by serum in myoblasts, whereas it enhanced the mitogenic response to serum in reserve cells. By employing selective S1P receptor agonist and antagonists, the co-mitogenic action of S1P in reserve cells was shown to depend mainly on S1P1. Real time PCR analysis revealed distinct S1P receptor pattern expression, which however could not account for the differential action of S1P in C2C12 reserve cells and myoblasts, thereby suggesting that the cell-specific responsiveness to S1P may depend on a different functional coupling of S1P1. This study discloses a unique pleiotropic effect of S1P which stimulates proliferation of muscle resident stem cells, such as reserve cells, and favours the growth arrest of committed progenitors cells, such as myoblasts, required for their subsequent myogenic differentiation.

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Abbreviations

S1P:

Sphingosine 1-phosphate

DMEM:

Dulbecco’s modified Eagle’s medium

FCS:

Fetal calf serum

BSA:

Bovine serum albumin

PBS:

Phosphate buffer saline

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Acknowledgements

We are indebted to Dr. F. Torricelli and Dr. B. Minuti (Cytogenetics and Genetics Unit, Azienda Ospedaliera Careggi, Florence, Italy) for skilful assistance with real time PCR experiments, and with Dr. R. Caporale (Department of Laboratory Medicine, Azienda Ospedaliera Careggi, Florence, Italy) for the help with cell cycle analysis. This work was supported in part by funds from the University of Florence and Fondazione Cassa di Risparmio di Pistoia e Pescia.

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Authors and Affiliations

  1. Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy

    Elena Rapizzi, Chiara Donati, Francesca Cencetti, Paola Nincheri & Paola Bruni

  2. Istituto Interuniversitario di Miologia (IIM), Università degli Studi di Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy

    Elena Rapizzi, Chiara Donati, Francesca Cencetti & Paola Bruni

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  1. Elena Rapizzi
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  2. Chiara Donati
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  3. Francesca Cencetti
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  4. Paola Nincheri
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  5. Paola Bruni
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Correspondence to Paola Bruni.

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Rapizzi, E., Donati, C., Cencetti, F. et al. Sphingosine 1-phosphate differentially regulates proliferation of C2C12 reserve cells and myoblasts. Mol Cell Biochem 314, 193–199 (2008). https://doi.org/10.1007/s11010-008-9780-y

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  • DOI: https://doi.org/10.1007/s11010-008-9780-y

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